摘要
为探讨半枝莲对Hela细胞胞内游离钙浓度的影响及其抗癌作用机理。利用荧光染料Fura 2在细胞内能与游离钙结合 ,在一定波长光的激发下可发出荧光的特性 ,根据荧光的强度来检测游离钙的变化 ,结果显示半枝莲对Hela细胞增殖的抑制作用不明显 ,但可通过促进细胞内储藏钙的释放和胞外钙离子的内流 。
To study the influence of Scutellaria barbata D Don on cytoplasmatic free Ca 2+ concentration of cervix cancer cells and its mechanism on anti cancer. Fluorochrome Fura 2 could combine with free Ca 2+ and then give off fluorescent under the condition of stimulating by some wavelength light The changes of free Ca 2+ were tested according to the intensity of fluorescent The results showed that Scutellaria barbata D Don could stimulate the cytoplasmatic store calcium out flow and extracellular Ca 2+ inflow, and could obviously improve the concentration of cytoplasmatic free Ca 2+
出处
《中药材》
CAS
CSCD
北大核心
2003年第10期730-733,共4页
Journal of Chinese Medicinal Materials
基金
福建省卫生厅青年基金资助项目 (3 2 0 2 1980 2 1#)
关键词
半枝莲
宫颈癌
抗癌药
游离钙
HELA细胞
Scutellaria barbata D Don
Cervix cancer cells
Free cytoplasmatic calcium
