发育期慢性染铅大鼠脑组织中NO、SOD、MDA的变化及其相互关系

Chang of NO、SOD、MDA in lead-treated rat brain and their correlation

目的 观察发育期慢性铅染毒大鼠脑组织中一氧化氮 (NO)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)的变化及其与血铅的剂量 -效应关系。方法 建立慢性铅染毒模型 ,隔日灌服醋酸铅 ,约 4周 ,观察各组血铅浓度与脑组织中NO、SOD、MDA的剂量 -效应关系以及NO、SOD、MDA 3者之间的相互关系。结果 (1)实验组与对照组血铅浓度间差别有显著性意义 (P <0 0 0 1)。 (2 )随着血铅浓度的升高脑组织中NO、MDA均呈上升趋势 (相关系数分别为 0 80 4、 0 70 3)。 (3)NO与SOD之间为负相关 (γ =- 0 95 5 ,P <0 0 0 1) ,SOD与MDA之间为负相关 (γ =- 0 85 ,P<0 0 0 1) ,NO与MDA之间为正相关 (γ=0 935 ,P<0 0 0 1)。结论 大鼠在慢性染铅过程中可导致脑组织中NO含量上升 ,SOD活性下降 ,MDA含量升高且呈剂量 -效应关系 ,推测可能是由于铅中毒诱导了iNOS而使NO含量增加 ,而SOD的活力下降 ,使体内蓄积了大量的O2 ,NO与O2 反应的结果生成了过多的氧化性极强的物质 。

Objective To study the chang of nitrc oxide(NO),super oxide dismutase(SOD) and malondialdehyde(MDA) in rat brain after lead exposure and the relationship among them.Methods Creat the lead exposure model by feeding PbAc for four weeks.Results There were significant differences in blood lead between lead-treated group and control group(P<0.001).There was positive dose-effece correlation between blood lead and NO , MDA in brain(γ=0.804,0.703),while there exist negative correlation (γ=-0.955,P<0.001) between NO and SOD,negative correlation(γ=-0.85,P<0.001) between SOD and MDA,positive correlation(γ=0.935,P<0.001) between NO and MDA.Conclusion NO content in brain increased SOD activity decreased and MDA content increased after chronic lead exposure,and there was does-effect relationship,between lead in blood and them,while there are correlation amony NO,SOD and MDA.It was indicated that NO content increase was the important result which damaged the brain of rat in lead poisoning.