长柄梭罗细胞毒活性部位的化学成分研究

Studies on chemical constituents of cytotoxicity portion in bark of Reevesia longipetiolat

目的 对长柄梭罗 Reevesia longipetiolata树皮具细胞毒活性的醋酸乙酯部分化学成分进行研究。方法 采用常压、加压硅胶柱色谱、Sephadex L H- 2 0凝胶柱色谱、高效液相色谱进行分离和纯化 ,通过理化和波谱分析方法鉴定化合物结构。结果 从其醋酸乙酯部分分离得到 5个化合物 ,分别鉴定为β-谷甾醇 (β- sitosterol, )、胡萝卜苷 (daucosterol, )、白桦脂酸 (betulinic acid, )、羽扇豆醇 (lupeol, )和 (+) -儿茶素 [(+) - catechin, ]。结论 5个化合物均为首次从该属植物中分得 ,并分别讨论了它们的细胞毒活性。

Object To study the chemical constituents of cytotoxicity portion in the bark of Reevesia longipetiolata Merr et Chun Methods The constituents were isolated and purified by various chromatographic methods, such as gel column chromatography under normal pressure and increased pressure, Sephadex LH 20 column chromatography and HPLC, and the structures were identified by physicochemical properties and spectral analysis Results Five compounds were obtained in the ethyl acetate fractions and identified as β sitosterol (Ⅰ), daucosterol (Ⅱ), betulinic acid (Ⅲ), lupeol (Ⅳ) and (+) catechin (Ⅴ) Conclusion All above compounds are obtained from the plants of Reevesia Lindl for the first time, and their cytotoxicity is discussed