皱落念珠菌临床分离株的基因型与表型差异性分析

Discrepancy of clinical isolates of Candida rugosa between genotype and phen otype

目的 分析皱落念珠菌临床分离株的基因型与表型的差异性。方法 利用来源于真菌保守区核糖体基因和可变区内转录间隔区 (ITS)基因为靶序列 ,应用通用引物扩增 13株非白念珠菌临床分离株基因组DNA ,并将该PCR产物进行克隆、序列分析鉴定 ,将鉴定为皱落念珠菌的临床分离株进一步作表型 (CHROM念珠菌显色培养及API 2 0CAUX真菌鉴定系统 )分析。结果 有 2株临床分离株基因型鉴定为皱落念珠菌 ,两者间同源性为 10 0 % ,与皱落念珠菌标准菌株 (ATCC 10 5 71T)基因序列的同源性为 95 %。但是 ,通过CHROM显色培养基及API 2 0CAUX系统进行表型分析 ,却均与热带念珠菌表型完全一致。

Objective To compare discrepancy between genotype and pheotype of th e clinical isolates of Candida rugosa. Methods The fung us-specific universal prim ers derived from the internal transcribed spacer (ITS) region of fungal rDNA wer e used for amplification. Genomic DNA purified from the thirteen clinical isolat es of non-C. Albicans was amplified by PCR. The purified PCR product was cloned into pBluescript Ⅱ KS(+) T vector and sequenced by Sanger's dideoxy chain terminatio n composition method. The two isolates were evaluated against CHROM Candida medi um and API 20C AUX. Results The two isolates of Candida rugosa were evaluated as Candida tropicalis by CHROM Candida medium and API 20C AUX. Conclusio ns Discrep ancy between genotype and phenotype of the two clinical isolates of Candida ru gosa was confirmed.