严重急性呼吸综合征患者外周血病毒载量和抗体水平动态监测

Dynamic changes of viral load and antibodies level in the patie nts of severe acute respiratory syndrome

目的 动态监测严重急性呼吸综合征 (SARS)患者外周血SARS病毒载量和抗体含量变化。方法 选择 3例因同一传染源感染的SARS患者为研究对象 ,应用实时荧光定量逆转录 聚合酶链反应 (RT PCR)检测患者外周血PBMCs中病毒载量 ,用酶联免疫检测技术检测血清SARS病毒IgG、IgM含量。 结果 3例患者分别在出现症状后第 3～ 11日、第 1～ 13日、第 1～ 9日检测到SARS冠状病毒核酸 ,第 5～ 7日病毒载量达到高峰。IgM抗体在患者起病后第 7～ 9日即可被检测到 ,IgG抗体在患者起病后第 7～ 14日即可被检测到 ,高峰在第 10～ 2 0日。结论 SARS病毒载量存在自限性消长过程。SARS抗体IgG、IgM均较早出现 ,IgG维持时间比IgM长。

Objective In order to study dynamic changes of viral load and antibodies level in SARS cas es. Methods Three SARS patients infected by the same infectious source were selected. Real-time fluorescence RT-PCR technology was used to quantify the SARS virus i n PBMCs, and ELISA technology was used to detect the level of anti-SARS IgG, IgM. Results The detectable periods for the SARS-CoV RNA were 3～11,1～13,1～9 days post-on set of the diseases, separately. Viral load peak emerged at 5～7 days 1.23×10 5 copies/ml～2.86×10 5 copies/ml. IgM, IgG antibodies became de tectable till 7～9 days and 7～14 days post -onset of the diseases, respectively. IgG antibody peak emerged at 10～20 days. Conclusion SARS-CoV load existed self-limited up to down course. Anti-SARS antibodies Ig G and IgM turn up in the early period, while IgG maintained longer than IgM.