构建含黑色素细胞组织工程皮肤的研究

CONSTRUCTION OF TISSUE ENGINEERING SKIN CONTAINING MELANOCYTES

目的 探讨运用组织工程方法构建含有黑色素细胞的组织工程皮肤。 方法 以包皮组织作为细胞来源 ,采用消化法获得角朊细胞、成纤维细胞和黑色素细胞 ,在自行设计的组织工程皮肤培养系统构建组织工程皮肤。行Dopa染色、透射电镜和 S- 10 0免疫组织化学检测构建的皮肤中黑色素细胞的分布和状态。 结果 构建的组织工程皮肤结构完整 ,细胞状态良好 ,Dopa染色、透射电镜和 S- 10 0免疫组织化学检测均显示含有大量黑色素细胞并处于良好状态。 结论 构建了含黑色素细胞的组织工程皮肤 ,可用于下一步的动物实验和临床实验。

Objective To construct a tissue engineering skin containing melanocytes by employing tissue engineering method. Methods The keratinocytes, dermal fibroblasts and melanocytes were isolated and purified. Then the cells were used to construct a tissue engineering skin containing melanocytes. The location of melanocytes in the tissue engineering skin were detected by Dopa staining, transmission electron microscope (TEM) and S-100 immunohistochemical staining.Results Melanocytes can be detected in the basal layer of the constructed tissue engineering skin. The results of TEM showed that the melanocytes were in good conditions. Conclusion The artificial skin containing melanocytes was successfully constructed in vitro and can be used to repair the full-thickness skin defects.