病理性瘢痕中结缔组织生长因子基因的表达

EXPRESSION OF CONNECTIVE TISSUE GROWTH FACTOR GENE IN THE HYPERTROPHIC SCAR AND KELOID TISSUE

目的 探讨结缔组织生长因子在增生性瘢痕和瘢痕疙瘩发病机制中的作用。 方法 分别留取正常皮肤 5例、增生性瘢痕 15例和瘢痕疙瘩 7例组织标本 ,测定组织中羟脯氨酸含量 ,通过逆转录 -聚合酶链反应 (RT- PCR)和图像定量分析 ,检测组织中结缔组织生长因子基因表达水平。 结果 正常皮肤、增生性瘢痕和瘢痕疙瘩组织中羟脯氨酸含量分别为 (2 6 .5 2± 4 .10 )、(84 .10± 1.76 )和 (92 .38± 2 .0 4 )μg/ mg,后两者与前者比较差异有统计学意义 (P<0 .0 1) ;正常皮肤、增生性瘢痕和瘢痕疙瘩组织中结缔组织生长因子 m RNA指数分别为 0 .0 9± 0 .2 5、0 .78± 0 .6 3和0 .84± 0 .0 4 ,后两者与前者比较差异有统计学意义 (P<0 .0 1)。 结论 结缔组织生长因子在增生性瘢痕和瘢痕疙瘩纤维化进程中可能起着促进作用。

Objective To explore the effect of connective tissue growth factor on the pathogenesis of hypertrophic scar and keloid tissue. Methods The content of hydroxyproline was determined and the expression of connective tissue growth factor gene was detected by the reverse transcription-polymerase chain reaction and image analysis technique in 5 normal skins, 15 hypertrophic scars and 7 keloid tissues. Results The contents of hydroxyproline in the hypertrophic scar(84.10±1.76) and keloid tissue (92.38±2.04) were significantly higher than that of normal skin tissue (26.52±4.10) (P<0.01). The index of connective tissue growth factor mRNA in the hypertrophic scar (0.78±0.63) and keloid tissue (0.84±0.04) were higher than that of normal skin tissue (0.09±0.25) (P<0.01). Conclusion Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar and keloid tissue.