中药复方TCMP-1对急性胰腺炎血小板内皮细胞粘附分子-1表达变化的研究

Effects of Chinese medicine on changes of platelet endothelial cell adhesion molecule-1 expression in acute edematous pancreatitis

目的 探讨急性水肿性胰腺炎(AEP)血小板内皮细胞粘附分子-1(PECAM-1)表达的变化规律及中药复方TCMP-1对AEP的治疗作用。方法 Wistar大鼠54只,诱发AEP动物模型,并给予TCMP-1干预,用流式细胞仪分析脾静脉血中多形核白细胞(PMN)PECAM-1的表达。结果 (1)在AEP自然病程组中,外周循环和胰腺微循环PMN PECAM-1的表达水平在AEP 2、4 h组相近,自AEP 4 h组开始,外周循环PECAM-1的表达上调直至AEP 8 h组;胰腺微循环PECAM-1的表达下调直至AEP 8 h组;在AEP 8 h组,PMN PECAM-1的表达有显著性差异(P<0.05)。(2)在AEP 8 h治疗组与AEP 8 h自然病程组中,体循环PMN PECAM-1的表达有显著性差异(P<0.05)。结论 (1)下调体循环PMN PECAM-1的表达水平或抑制PMN PECAM-1的过度表达,可以阻止胰腺微循环中PMN与内皮细胞的粘附。(2)抑制PMN PECAM-1的过度表达是中药复方TCMP-1实现其疗效作用的主要机制之一。

Objective To study the effects of Chinese on changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression in acute edematous pancreatitis (AEP). Methods After the model of AEP was established in 54 Wistar rats, the changes of PECAM-1 expression on leukocytes in splenic vein and inferior vena cava was determined by flow cytometry. Results (1) PECAM-1 expression on PMN had no significant difference between pancreatic microcirculation and systemic circulation in AEP 2 h group and AEP 4 h group. It in pancreatic microcirculation began to decrease in AEP 4 h group and was continuously down-regulated in AEP 8 h group while that in systemic circulation began to increase in AEP 4 h group and was continuously up-regulated in AEP 8 h group. Furthermore, it was significantly different between pancreatic microcirculation and systemic circulation in AEP8h group (P<0. 05). (2) Significant difference was found in systemic circulation between TC-MP-1 treatment groups and AEP group (P<0. 05). Conclusions (1) The down-regulation of PMN PECAM-1 expression in systemic circulation may prevent PMN from adhering to endothelial cells. (2) The inhibition of over-expression of PMN PECAM-1 may be one o the main effect of TCMP-1 in treatment of AEP.