Bcl-2基因反义核酸增强三氧化二砷对NB4细胞凋亡效应

The antisense phosphorothioate oligodeoxynucleotides of bcl-2 oncogene enhances the apoptotic effect of As_2O_3 on NB4 leukemic cells

目的 :研究和探索bcl- 2基因反义核酸 (bcl- 2ASODN)是否能提高三氧化二砷 (As2 O3 )对NB4白血病细胞的凋亡效应。方法 :采用微量细胞培养的方法 ,观察反义核酸、砷剂单独以及反义核酸、砷剂联合培养NB4细胞的生物效应和凋亡形态变化 ;用免疫组化的方法 ,结合流式细胞技术 ,测定NB4细胞的DNA和Bcl- 2蛋白的变化。结果 :As2 O3 (0 2 5 μmol/L) +bcl - 2ASODN(10 0 μmol/L)联合诱导NB4细胞凋亡作用显著强于单用As2 O3 (0 2 5μmol/L)或 bcl- 2ASODN(10 0 μmol/L) (P <0 0 1)。流式细胞仪检测显示 ,联合用药Bcl- 2蛋白表达亦明显少于反义核酸、砷剂单独给药组 (P <0 0 1)。结论 :bcl- 2基因反义核酸能明显提高和显著增强As2 O3 对NB4白血病细胞的致凋亡效应。

AIM: To study and explore whether the antisense phosphorothioate oligodeoxynucleotides (ASODN) of bcl-2 oncogene would increase the apoptotic effect of As 2O 3 on NB4 leukemic cells. METHODS: The biological and morphological changes in NB4 cells from microculture with As 2O 3, bcl-2 ASODN or both were observed. The changes in DNA content and Bcl-2 protein of NB4 cells from microculture with As 2O 3, bcl-2 ASODN or both were determined by tissue chemistry and flowcytometry. RESULTS: There was much more apoptotic effect of As 2O 3 on NB4 cells while it combined with bcl-2 ASODN (ASODN 10 0 μmol/L+ As 2O 3 0 25 μmol/L) than alone(ASODN 10 0 μmol/L or As 2O 3 0 25μmol/L),and so did inhibitory effect of Bcl-2 protein expression by flow cytometry. CONCLUSION: The bcl-2 ASODN can enhance the apoptotic effect of As 2O 3 on NB4 leukemic cells. [