共轭型亚油酸对B(a)P诱导小鼠前胃癌的抑制作用:150001黑龙江省哈尔滨医科大学营养与食品卫生教研室

The inhibitory effect of CLA on mice forestomach neoplasia induced by B(a)P

目的 研究共轭型亚油酸 (CAL)的抑癌作用 ,并为进一步探讨CLA的抑癌机制提供线索。方法 以B(a)P诱导建立小鼠前胃癌动物模型 ,观察CLA对小鼠前胃癌促长阶段的抑制效果 ,共分 5组 ,即色拉油阴性对照组、CLA阴性对照组、B(a)P阳性对照组、B(a)P +CLA高剂量实验组和B(a)P +CLA低剂量实验组。整个实验期为 2 3周 ,检测细胞增殖、Pan rasP2 1蛋白表达和氧化还原酶等指标。结果 短期给予CLA对B(a)P诱导的小鼠前胃癌促长阶段具有明显的抑制作用 ,B(a)P阳性对照组、B(a)P +CLA高剂量实验组和B(a)P +CLA低剂量实验组的肿瘤发生率分别为 10 0 %、6 0 %和6 9 %。其抑癌机制与其对细胞增殖活力的抑制及对机体GSH Px、GST和SOD酶活力的诱导作用有关 ,而并不依赖于对Pan rasP2 1蛋白表达的调节途径。结论 提示共轭型亚油酸是一种很有前途的化学预防剂 。

Objective To study the inhibitory effect of CLA on the postinitiation phase of forestomach neoplasia formation induced by B(a)P and explore its possible mechanism of anticarcinogenesis Methods Kunming mice were divided into five groups, i e salad oil control group, CLA control group, B(a)P group, B(a)P+high dose CLA group and B(a)P+low dose CLA group The experimental period was 23 weeks, and the endpoints included cell proliferation, expression of Pan ras P21 and some enzymes Results Short term CLA treated significantly inhibited the forestomach neoplasia formation induced by B(a)P at postinitiation phase The incidence of tumor in the groups of B(a)P, B(a)P+high dose CLA and B(a)P+low dose CLA was 100%, 60% and 69% respectively ( P <0 05) The possible mechanism of this anticarcinogenic effect may be related to the inhibition of cell proliferation and the induction of the activities of GSH Px, GST and SOD, irrespective of the regulation of expression of Pan ras P21 Conclusion CLA is a promising chemopreventive agent, and its anticarcinogenic effect may involve its effects on the redox system