摘要
Purpose: Dexamethasone(DEX) was tested for its ability to modulate human retinal pigment epithelium (hRPE) cell proliferation in cell culture.
Methods: DEX in different concentrations was added to cultured hRPE cells. The effects were measured with MTT method, 3H-thymidine(3H-TdR) incorporation and flow cytometw.
Results: DEX increased survival rate and DNA synthesis from 32 mg/L to 320 mg/L under hRPE culture conditions, but paradoxically reduced them at 1 000 mg/L and 3 200 mg/L in dose and time dependent fashion by both MTT assay and 3 H-TdR incorporation. The cell numbers in S phase and G2/M phase increased 28.32 % at DEX concentration 320 mg/L, in contrast, reduced 41.84 % at 1 000 mg/L.
Conclusion: DEX increased proliferation from 32 mg/L to 320 mg/L, and inhibited proliferation at concentrations greater than 320 mg/L. The inhibiting effect of DEX may happen in s phase and G2/M phase. Eye Sciernce 2001; 17:27 ～ 30.
Purpose: Dexamethasone(DEX) was tested for its ability to modulate human retinalpigment epithelium (hRPE)cell proliferation in cell culture.Methods: DEX in different concentrations was added to cultured hRPE cells. The effectswere measured with MTT method, 3H-thymidine(3H-TdR) incorporation and flowcytometry.Results : DEX increased survival rate and DNA synthesis from 32 mg/L to 320 mg/Lunder hRPE culture conditions, but paradoxically reduced them at 1 000 mg/L and3 200 mg/L in dose and time dependent fashion by both MTT assay and 3H-TdRincorporation. The cell numbers in S phase and G2/M phase increased 28. 32 % at DEXconcentration 320 mg/L, in contrast, reduced 41. 84 % at 1 000 mg/L.Conclusion: DEX increased proliferation from 32 mg/L to 320 mg/L, and inhibitedproliferation at concentrations greater than 320 mg/L. The inhibiting effect of DEX mayhappen in s phase and G2/M phase.
