摘要
目的 :研究苦参碱 (Matrine,简称 Mat)对人增生性瘢痕成纤维细胞增殖的影响。 方法 :将 Mat以不同浓度梯度作用于体外培养的增生性瘢痕成纤维细胞 ,测MTT反应的 A0值及 L DH值 ,用免疫组织化学检测 Mat用药前后的细胞核内增殖性抗原 (proliferating cell nuclear antigen,PCNA)的表达。 结果 :增生性瘢痕成纤维细胞在 Mat0 .2 5～ 10 0 m g/m l范围内呈剂量依赖性增殖抑制 ,但 L DH值无明显改变 ;PCNA的表达 ,在 Mat作用后明显减弱。 结论 :Mat在体外能明显抑制增生性瘢痕成纤维细胞的增殖而不引起细胞坏死性改变。
Objective:To study the influence of Matrine on growth and proliferation of fibroblasts derived hgpertropnic scar. Methods:The MTT OD value and the content of LDH was examined by biochemical analysis after Matrine was added to the monolayer culture system of scar derived fibroblasts.The expressions of prolifrating cell nuclear antigen (PCNA)were examinedby immunochemied methed. Results:Mat at 0 10～100 mg/ml could decrease the MTT OD value in dose dependent manner amd could not increase the content of LDH significantly.The expression of PCNA in Mat groupswas highr than the control groups. Conclusion:Mat could inhibit the proliferation of derived hypertropnicscar fibroblasts rarely produce the toxicity on scar derived fibroblasts. [
出处
《中国美容医学》
CAS
2001年第1期6-8,F003,共4页
Chinese Journal of Aesthetic Medicine
基金
沈阳军区重点科技项目
