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不同DNA分子标记技术信息量比较 被引量:11

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摘要 本文对RFLP、RAPD、SSR和AFLP四种主要的分子标记在研究多态性时所揭示的信息量进行比较研究,结果表明:(1)SSR标记的多态性最高,多态性信息含量PIC值最大,适于品种指纹图谱绘制、基因型鉴定和核心种质构建。另外,由于SSR标记主要检测基因组的重复区,因此用SSR标记研究功能基因区的差异效果较差;(2)AFLP标记评价效率最高,评价效率指数Ai值最大,在一次扩增中能有上百条带,不但能比较不同样品的相异的带,而且可以从相同的带型及带的多少来比较遗传背景的相似程度,适于研究不同种质问的遗传关系、亲子间的系谱分析等。
出处 《植物遗传资源科学》 CSCD 2000年第4期41-46,共6页
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参考文献20

  • 1许占友,邱丽娟,常汝镇,李向华,郑翠明,刘立宏,郭蓓.利用SSR标记鉴定大豆种质[J].中国农业科学,1999,32(S1):40-48. 被引量:43
  • 2胡志昂,恽锐,钟敏,董夫贵,王洪新,钱迎倩.检测植物DNA扩增多态性方法的比较和改进[J].Acta Botanica Sinica,1997,39(2):144-148. 被引量:22
  • 3黎裕,贾继增,王天宇.分子标记的种类及其发展[J].生物技术通报,1999,15(4):19-22. 被引量:158
  • 4Bohn M et al. 1999. Genetic similarities among winter cultivars determined on the basis of RFLPs, AFLPs, SSRs and their use for predicting progeny variance. Crop Sci. 39:228-237.
  • 5Chavarriaga-Aguirre P et al. 1999. Using microsatellite, isozyme and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-based markers to maintain germplasm collections. Molecular Breeding. 5 : 263- 273.
  • 6Hallden C et al. 1994. Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines. Theor. Appl. Genet. 88:123-128.
  • 7Lin J J et al. 1996. Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques. Plant Molecular Biology Reporter. 14(2):156-169.
  • 8Liu Z et al. 1993. Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and higtooth aspen. Theor. Appl. Genet. 87:97-105.
  • 9Lu J et al. 1996. Comparative analysis of genetic diversity in pea .assessed by RFLP and PCR-based methods. Theor. Appl. Genet. 93:1103-1111.
  • 10Maughan P J et al. 1995. Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean. Genome. 38:715-723.

二级参考文献16

  • 1李 莹等.大豆遗传资源研究论文集[M]山西科学技术出版社,1991.
  • 2G. Wang,R. Mahalingam,H. T. Knap.(C-A) and (G-A) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr.[J]. TAG Theoretical and Applied Genetics . 1998 (8)
  • 3J. Rongwen,M. S. Akkaya,A. A. Bhagwat,U. Lavi,P. B. Cregan.The use of microsatellite DNA markers for soybean genotype identification[J]. Theoretical and Applied Genetics . 1995 (1)
  • 4Morgante M,etal.Genetic m apping and variability ofseven sim plesequencesrepeatlociin soybean. Genome . 1994
  • 5Cregan PB,etal.Microsatellite fingerprinting and m apping ofsoybean.Methods Mol. CellBio . 1997
  • 6Akkaya M S,et al.Length polym orphism ofsim ple sequence repeats in soybean. Genetics . 1992
  • 7Maughan P J,et al.Microsatellite and am plified sequence length polym orphism s in cultivated and w ild soybean. Genome . 1995
  • 8Akkaya M S,etal.Integration ofsim ple sequence repeatsm arkers into a soybean linkage m ap. Crop Science . 1995
  • 9G. Caetano-Anollés. MAAP: a versatile and universal tool for genome analysis[J] 1994,Plant Molecular Biology(6):1011~1026
  • 10Brant J. Bassam,Gustavo Caetano-Anollés. Silver staining of DNA in polyacrylamide gels[J] 1993,Applied Biochemistry and Biotechnology(2-3):181~188

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