肉桂酸对A549细胞分化及端粒酶活性的影响

Effects of cinnamic acid on telomerase activity and cell differentiation in A549 cells

目的 :探讨肉桂酸对人肺腺癌A5 4 9细胞诱导分化及对端粒酶活性的影响。方法 :应用 1mmol/L ,3mmol/L肉桂酸 (CINN )分别处理A5 4 9细胞 ,并设空白对照 ,观察体外细胞增殖情况 ,细胞分裂指数 ,流式细胞仪测定细胞周期 ,以TRAP -银染技术的非放射性方法检测端粒酶活性。结果 :CINN作用后的A5 4 9细胞 ,细胞增殖明显受到抑制 ;细胞分裂指数减少 ;细胞周期出现向G1 /G0期移行的特征性动力学改变 ;相应的端粒酶活性下降 ,甚至活性丧失。结论 :肉桂酸能诱导A5 4

Aim:To study the differentiation effect of cinnamic acid (CINN) on human lung adenocarcinoma A549 cells and the possible mechanism. Methods: A549 cells were treated by CINN (1mmol/L, 3mmol/L), the cell growth rate was determined by cell counting, and its viability was determined by trypan-blue exclusion assay. Flow cytometry (FCM) was used to analyze the cell cycle. The telomerase activity was detected by the non-radioactive method (silver staining method) during the course of differentiation of A549 cell line.Results:After A549 cells were incubated with CINN, the cell proliferation was markedly inhibited; the cell division index of the CINN groups was lower than that of the control group; cell cycle showed G1/G0 phase stasis; the telomerase activity in different CINN groups changed markedly. Conclusion:CINN could inhibit the proliferation of A549 cells and the telomerase activity.