乳腺肿瘤组织中NOS活性、NO及MDA含量检测

Detection of nitric oxide synthese activity, content of NO and MDA in human breast tumor

目的 :检测乳腺肿瘤组织中一氧化氮合酶 (NOS)活性 ,一氧化氮 (NO)和丙二醛 (MDA)的含量。方法 :应用分光光度计法分别检测乳腺良 (2 0例 )、恶性 (2 8例 )肿瘤组织中NOS活性、NO及MDA的含量。结果 :乳腺恶性肿瘤组织中NOS活性 ((0 .4 3± 0 .2 8)nmol/(min·g) )与良性肿瘤组织 ((0 .2 1± 0 .1 1 )nmol/(min·g) )相比明显增高 (P <0 .0 5 ) ,而MDA含量 ((3.3± 1 .4 ) μmol/g)vs((4 .5± 0 .2 ) μmol/g)却明显降低 (P <0 .0 5 ) ;NO含量在恶性肿瘤组织中虽有一定增高 ,但差异无统计学意义 (P >0 .0 5 )。在雌激素受体 (ER) (- )乳腺恶性肿瘤组织中 ,NOS活性明显高于ER(+)者 (P <0 .0 5 )。结论

Aim: To detect nitric oxide (NO), malondialdehyde (MDA), and NOS activity in human breast tumors. Method: To examine NO, MDA, and NOS activity in 48 cases of human benign and malignant breast tumors by spectrophotometry. Result:The activity of NOS was significantly higher in malignant tumor tissues((0.43±0.28)nmol/(min·g)) than that in benign tumor tissues ((0.21±0.11)nmol/(min·g), P <0.05), but the content of MDA was significantly lower than that in benign tumor tissues((3.3±1.4)μmol/g)vs((4.5±0.2)μmol/g). Although the content of NO was higher in malignant tumor tissues, there was no significant difference between 2 groups ( P >0.05). In malignant tumor tissues, the activity of NOS was significantly higher in cases with ER (-) than those with ER (+). Conclusion: It was suggested that the increase of NOS activity might promote the development of breast cancer.