中国亚热带常见园林植物清除DPPH自由基活性研究

Studies on Scavenging Activity Against DPPH Free Radical in Fresh Leaves from Some Common Subtropical Plants of Garden and Afforestion in China

采用二苯基苦基苯肼 (DPPH)自由基酶标仪法 ,对中国亚热带常见的 83科 16 4种园林绿化植物鲜叶的自由基清除活性进行了分析比较 ,结果表明 ,清除活性强 (清除 50 %DPPH自由基时的样品浓度IC50 <0 50 0mg·mL-1 )的有 2 5种 ,清除活性中等 (IC50 0 50 1～1 0 0 0mg·mL-1 )的 31种 ,清除活性弱 (IC50>1 0 0 1mg·mL-1 )的 10 8种。其中石榴、金缕梅、枫香、三角枫、木、算盘子、山杜英、青冈栎、棣棠、南天竹等 10种植物鲜叶DPPH自由基清除活性IC50 <0 4 0 0mg·mL-1 ,其它种IC50 值介于 0 4 0 1～ 14 30 0mg·mL-1 之间。

A DPPH-Microplate assay was made to determine the f re e radical scavenging activity in leaves of subtropical plants of garden and affo restion belonging to 83 Families 164 Species. The results showed it can be divid ed into three types of high(sample concentration of scavenging 50 percent of DPP H free radical IC 50 <0 500mg·mL -1 ),medium(IC 50 0 50 1-1 000mg·mL -1 )and low(IC 50 >1 001mg·mL -1 )free radical scavenging activity. There are 25 with high activity,31 with medium,10 8 with low. Among them,Pomegranate,Chinese Witchhazel,Beautiful Sweetgum,Bue rger Maple, Chinese Loropetalum,Puberulous Glochidion,Sylvestral Elaeocarpus, Blue Japanese Oak,Japanese Kerria and Common Nandina have the highest activity. Their IC 50 are 0 283、0 292、0 312、0 326、0 345、0 369、0 379、0 389、0 398、0 403mg·mL -1 ,respectively. Others IC 50 is between 0 401～14 300 mg·mL -1 . It is suggested that these species can b e used a new resource of free radical scavenger and antioxidant products.