中国樱桃‘对樱桃’不定根离体再生植株的研究

Plant Regeneration from in Vitro Adventitious Roots of Chinese Cherry ‘Duiyin gtao’ (Prunus pseudocerasus)

采用中国樱桃‘对樱桃’ (Prunuspseudocerasus)无菌生根苗的不定根作为外植体 ,成功诱导了胚性愈伤组织 ,实现了通过分化不定芽和诱导初生体细胞胚两种方式再生植株。根切段在MS +NAA1 0mg/L +BA 0 0 5mg/L +IBA 0 0 5mg/L培养基诱导获得胚性愈伤组织 ,诱导频率达 54 2 % ,该愈伤组织在MS +NAA 1 0mg/L +BA 0 5mg/L培养基上不定芽分化率为 10 0 % ,不定芽在MS+BA 0 5mg/L +IBA 0 1mg/L培养基上生长发育良好 ,转入MS+NAA 0 0 2mg/L +IBA 0 0 2mg/L生根培养基生根率达 10 0 % ;整体不定根系统在MS附加 2 ,4 D 1 0～ 3 0mg/L和BA 0 5mg/L的培养基上同时诱导初生体细胞胚发生和单极不定芽发生 ,每外植体上平均体细胞胚数 5～ 2 5个 ,不定芽 3～ 2 2个。体细胞胚转到MS +BA 0 5mg/L +IBA 0 1mg/L培养基上发育为完整植株 ,植株再生率 10 0 %。

Plant regeneration was obtained on embryogenic callus,which de rived from in vitro adventitious roots of a Chinese cherry(Prunus pseudocerasu s)cultivar‘Duiyingtao’,either by adventitious bud proliferation or primary s omatic embryogenesis.Embryogenic callus were induced from root segments on mediu m MS+NAA 1 0mg/L+BA 0 05mg/L+IBA 0 05mg/L,with the fr equency of 54 2%.Adventitious bus proliferation emerged on all embryogenic call us on medium MS+NAA 1 0mg/L+BA 0 5mg/L,the buds developed on m edium MS+BA 0 5mg/L+IBA 0 1mg/L,and rooted on medium MS+NAA 0 02mg/L+IBA 0 02mg/L by 100%. Primary somatic embryos and a dventitious bud were induced from intact root systems simultaneously on medium M S+2,4-D 1-3mg/L+BA 0 5mg/L,with 5-25 somatic em bryos and 3-22 adventitious buds per root explant.100% of the somatic embryos de veloped into intact plantlets when transferred to medium MS+BA 0 5mg/L+ IBA 0 1mg/L.