尖锐湿疣患者Th1/Th2细胞因子的检测

Detection of Th1/Th2Cytokines in the Peripheral Blood Lymphocytes of Patients with Condyloma Acuminatum

目的检测尖锐湿疣(CA)患者CD3+T细胞分泌细胞因子的水平,分析Th1细胞因子和Th2细胞免疫活动。方法首先用刺激物刺激细胞,增加细胞内细胞因子的表达,然后加入荧光标记的特异性抗细胞因子单克隆抗体,特异性抗原抗体结合,最后应用流式细胞仪分析特异性细胞因子表达水平。结果CA患者Th1型细胞因子干扰素γ、白介素2、肿瘤坏死因子α、白介素12表达水平较正常对照显著降低,Th2型细胞因子白介素10表达水平较正常人对照差异无显著性。结论CA患者Th1型反应模式处于弱势状态,相对地Th2型反应模式处于优势状态,Th1/Th2平衡失调,Th1/Th2平衡向Th2方向漂移。CA患者体内Th2型细胞因子模式占优势状态,可能是病毒发生免疫逃逸引起免疫损伤的一种机制。

Objective To detect cytokine levels secreted by CD3 + T cells and monitor the Th cell im-balance in the patients with condyloma acuminatum(CA).Methods The peripheral blood lymphocytes were isolated and stimulated prior to analysis.Cytokine detection was performed using monoclonal antibodies,which were fluorochrome-conjugated for easier direct manipulation in flow cytometry analysis.The results were pre-sented as cytograms showing dual staining of lymphocytes.Results The levels of IL-2,IL-12,IFN-γand TNF-αproduced by Th1cells decreased,while the level of IL-10produced by Th2cells had no significant change in CA patients in comparison with healthy controls.Conclusions There is Th1/Th2imbalance in CA patients with a decline of Th1type cytokines.The Th1/Th2imbalance might be the mechanism that HPVs es-cape from immunosurveillance in CA patients.