梅毒患者病程中宿主的免疫学变化

Immunological Changes in Syphilitic Paitents with Different Courses

目的探讨梅毒螺旋体(TP)感染的不同病程与宿主免疫学变化的关系。方法采用ELISA法分别检测一期和二期梅毒患者血清抗TP抗体的滴度(用活动指数AI表示)。MTT比色法测定一期和二期梅毒患者血清对脐带血淋巴细胞增殖反应的影响(用刺激指数SI表示)。ABC鄄ELLSA法分别检测一期和二期梅毒患者血清中细胞因子IL鄄2、IL鄄4含量。结果二期梅毒患者抗TP抗体的活动指数(AI)显著高于一期梅毒(t=3.92,P<0.01)。一期和二期梅毒血清对脐带血淋巴细胞增殖反应的刺激指数SI显著低于无血清含PHA组(q1=12.99,P1<0.01。q2=12.04,P2<0.01),且二期梅毒血清SI亦显著低于一期梅毒(q=7.18,P<0.01)。一期梅毒血清中IL鄄2含量明显高于二期梅毒(t=3.50,P<0.05),IL鄄4含量与二期梅毒差异无显著性(t=1.31,P>0.05)。结论抗TP抗体随着梅毒病程由一期向二期进展其滴度越来越高,但与体液免疫有关的细胞因子IL鄄4没有发生显著性变化;宿主感染梅毒螺旋体后,其细胞免疫功能受到明显抑制,且随着病程由一期向二期进展,其抑制现象越来越显著。

Objective To study the relationship between the different courses of Treponema pallidum(TP)infection and the immunological changes of the host.Methods ELISA was used to detect the anti-TP antibody in the sera of patients with primary syphilis and secondary syphilis.The MTT chromatometry was used to detect the effect of syphilitic sera on the proliferation of lymphocyte from umbilical cord blood.ABC-ELISA was used to detect the serum level of IL-2,IL-4in syphilis patients.Results The activity index(AI)of anti-TP antibody in secondary syphilis was higher than that in primary syphilis(t=3.919,P<0.01).Stimulation index(SI)for lymphocyte proliferation in primary and secondary syphilis was significantly lower than that in control group(q 1 =12.99,P 1 <0.01.q 2 =12.04,P 2 <0.01).The SI in secondary syphilis group was significantly lower than that in primary group(q=7.18,P<0.01).The serum level of IL-2in primary syphilis was higher than that in secondary syphilis(t=3.50,P<0.05).The serum level of IL-4in primary syphilis was not different from that in secondary syphilis(t=1.31,P>0.05).Conclusions The anti-Treponema pallidum antibody significantly increases as the course progresses from primary syphilis to the secondary stage.It seems that the celluar immunity in syphilis patients is suppressed along with the progress of course from primary stage to secondary stage.