摘要
对猪脂肪组织cDNA文库的2104个克隆进行EST研究,其中563个ESTs在猪种中已有匹配序列,682个在人类及其它物种中可以找到同源序列,376个ESTs为未知新基因,有298个因序列质量差无法做分析,185个在EST库中有同源序列。所分析的376个新ESTs中,其中有159个具有ORF结构,43个具有Poly(A)和CpG特征,这些结果初步显示了猪脂肪组织的功能基因表达特点。
2 104 expressed sequence tags(ESTs) from a cDNA library of porcine adipocyte were studied. The results showed that the nucleotide sequences of 563 ESTs have already been presented in GenBank database, 682 ESTs could be found homologous with those of human and other species, while 376 ESTs were not identified. 298 ESTs were e-liminated for the poor quality of sequence, and 185 ESTs have their homologues in the EST database. Of the 376 ESTs, 159 have ORF structure, and 43 contain Poly (A) or/and CpG elements, and 174 had no above structures or elements. All these results preliminarily showed the expression style of functional genes in porcine adipocyte.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第10期1209-1212,共4页
Scientia Agricultura Sinica
基金
国家重大基础研究规划资助项目(G20000161)
