大肠癌组织及其癌旁粘膜PCNA和AgNORs的检测及其临床意义

The Clinical Study of PCNA and AgNORs in Colorectal Carcinoma and Carcinoma Adjacent Mucosa

目的 探讨大肠癌组织及癌旁粘膜中增殖细胞核抗原 (PCNA)的表达和核仁区嗜银染色 (AgNORs)计数的临床意义。方法 采用免疫组织化学 (SP法 )和嗜银染色技术对 48例大肠癌组织及其癌旁粘膜和 1 0例正常大肠粘膜中PCNA和AgNORs进行检测。结果 大肠癌组织中PCNA标记指数 (PCNA LI)和AgNORs计数均显著高于癌旁 3cm和 6cm粘膜及正常粘膜 (P<0 .0 1 ) ,PCNA LI在DukesC期和D期显著高于DukesA期 (P<0 .0 5)。癌旁 3cm粘膜细胞AgNORs计数显著高于癌旁 6cm粘膜 (P<0 .0 1 )及正常粘膜细胞的AgNORs计数 (P<0 .0 5)。结论 大肠癌癌旁粘膜部分细胞增殖调节失控 ,提示癌旁粘膜是一种不稳定的癌前状态 。

Objective To study the expression and significance of proliferating cell nuclear antigen (PCNA) and argyrophilia nucleolar organizer regions (AgNORs) in colorectal carcinoma (CRC) and carcinoma adjacent mucosa (CAM).Methods The expression of PCNA in 48 cases of colorectal carcinoma tissue, CAM and 10 cases of normal mucosa was detected by immunohistochemistry techniques. AgNORs was determined with argyrophilia stain. Results The PCNA labeling index (PCNA LI) and AgNORs count in CRC were higher than that in CAM and normal mucosa( P <0.01).The PCNA LI in Dukes C and D stage was higher than that in Dukes A( P <0.05). The AgNORs count in 3 cm CAM was higher than 6 cm CAM ( P <0.01) and normal mucosa( P <0.05). Conclusion Some cells proliferative activity were abnormal in CAM. It indicates that CAM is in an unstable premalignant state, which might have some correlation with the relapse of colorectal carcinoma.