环氧化酶-2和血管内皮生长因子在胰腺癌组织中的表达及其相关性研究

Cyclooxygenase-2 Expression and Its Correlation with Vascular Endothelial Growth Factor in Pancreatic Adenocarcinoma

目的 探讨环氧化酶 2 (COX 2 )和血管内皮生长因子 (VEGF)在胰腺癌组织中的表达及与其生物学行为的相关性。方法 采用免疫组织化学Envision法对 5 1例胰腺导管癌组织中COX 2和VEGF的表达进行检测。结果 该 5 1例胰腺导管癌组织中COX 2和VEGF的表达阳性率分别为 74.5 %和 68.6% ,而二者在 11例癌旁胰腺组织中均未见阳性表达 ;COX 2和VEGF的表达阳性率在临床Ⅲ～Ⅳ期明显高于临床Ⅰ～Ⅱ期 ,淋巴结转移阳性组明显高于淋巴结转移阴性组 ,其差异均有显著性意义 (P<0 .0 5 ) ;COX 2和VEGF的表达与胰腺癌的组织学分级、患者的性别、年龄以及肿瘤的大小和部位无关 (P>0 .0 5 ) ;COX 2的表达与VEGF的表达呈正相关 (r =0 .411,P<0 .0 1)。结论 COX 2和VEGF可能在胰腺癌的发生、发展过程中起着关键性作用 ,二者在血管生成过程中密切相关 ,可能为胰腺癌的治疗提供新的靶点 ,值得深入探讨。

Objective To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in human pancreatic adenocarcinoma and their correlation with clinicobiological behavior. Methods The expression of COX-2 and VEGF in 51 cases of human pancreatic ductal adenocarcinoma were detected with immunohistochemistry of Envision. Results Expression of COX-2 and VEGF in pancreatic ductal adenocarcinoma were 74.5 % and 68.6 %, respectively; no expression of COX-2 and VEGF in adjacent normal tissue was detected. Both COX-2 and VEGF expression in clinical stage Ⅲ-Ⅳ were much higher than those in clinical stage Ⅰ-Ⅱ, and also higher in positive group of lymph node metastasis than in negative group as well ( P < 0.05). None of them had relation with histological grades, age, sex, tumor size and location. The expression of COX-2 was closely correlated with VEGF ( r =0.411, P < 0.01). Conclusion COX-2 and VEGF may play a pivotal role in tumorigenesis and tumor progression in pancreatic cancer, they may provide new targets for therapy of pancreatic cancer.