新生儿念珠菌感染母婴垂直传播的分子流行病学研究

A Molecular Epidermiologic Study of Mother to Child Transmission of Candida Specis in Newborn

目的 :了解足月孕妇念珠菌感染的基本状况 ,及新生儿念珠菌感染的病原菌来源 ,为念珠菌感染防治提供依据。方法 :采用念珠菌培养、Germ -tube试验、革兰染色、API2 0CAUX念珠菌鉴定系统及DNA及指纹检测对 2 0 8对足月孕妇阴道分泌物、口腔标本及新生儿的口腔标本进行念珠菌分离及鉴定。结果 :母体阴道念珠菌检测阳性率 5 1.92 % ,口腔念珠菌检测阳性率 4 7.5 9% ,其中 2 0 .19% ,阴道与口腔同时阳性 ,初生婴儿口腔念珠菌的阳性检出率 0 .96 % ,足月新生儿口腔念珠菌的阳性检出度 2 9.35 %明显高于初生婴儿 (P <0 .0 1) ;仅有 7对母婴念珠菌检测均为阳性 ,但不具有相同的遗传特性。结论 :足月孕妇有较高的念珠菌感染率 ,新生儿念珠菌感染的病原菌来源以环境为主 。

Objective: To study the frequency of Candida infection of women in their third trimesters of pregnancy and to identify unique strains of Candida colonizing selected woman at deliver and new born offspring. Methods: 208 mother-infant pairs were investigated. The Candida species were identified by sugar assimilation formentation techniqes the germ tube test, API 20C AUX and DNA fingerprint technique. Results: The Candida species detetive rate from mother's vaginal was 51.92%, from oral was 47.59%,among them both from vaginal and oral was 20.19%. The oral of neonate in the 5 days was 0.96%,the one month old infant was 29.35%. 7 mother-infant pairs were Candida positive, but had different DNA fingprint. Conclusion: Candida species detective was highly in the women in their third trimester prognancy, The acquisition of Candida by neonates occurs via environmental sources.