肠三叶因子对新生鼠缺氧肠损伤模型白细胞介素8、丙二醛的影响及意义

The Effect and Significance of Intestinal Trefoil Factor IL-8 and MDA for Neonatal Rat Model for Hypoxia-induced Intestinal Injury

目的 研究肠三叶因子 (intestinaltrefoilfactor,ITF)对坏死性小肠结肠炎 (necrotizingenterocolitis,NEC)新生大鼠的肠粘膜中白细胞介素 8(interleukin 8,IL 8)及丙二醛 (malondialdehyde,MDA)含量的影响 ,探讨ITF对NEC是否有保护作用。 方法 建立NEC模型 ,对新生 1日龄Wistar大鼠予 10 0 %二氧化碳 ,5min后再予 10 0 %氧气 ,5min后放回母鼠身边喂养 ,第 4天处死大鼠取肠道组织待检。新生鼠 32只随机分为四组 ,A组为NEC模型后腹腔注射ITF 0 .5mg ;B组为NEC模型后皮下注射ITF 0 .2mg ;C组为NEC模型组 ;D组为正常对照组。取近回盲段 1～ 2cm肠道组织固定包埋、切片、HE染色光镜下作病理学检查 ,其他肠道组织制备组织匀浆取上清液用放射性免疫法检测IL 8,用生化法检测MDA的含量。 结果 A、B组IL 8的含量 [pg (mg·pro) ]各为 2 9.72 2± 7.134、30 .5 12± 8.2 30 ,均较C组 39.379± 4 .4 2 0低 (P <0 .0 5 ) ,而与D组 2 3.92 2± 6 .16 8比较 ,差异无显著性 (P >0 .0 5 ) ;A、B组MDA的含量 [nmol (mg·pro) ]各为 2 .2 6 7± 0 .2 6 7、2 .15 4± 0 .30 1,较C组 3.378± 0 .835低 (P <0 .0 1) ,与D组 2 .2 2 5± 0 .4 4 3比较 ,差异无显著性 (P >0 .0 5 )。C组HE染色切片见肠壁的损伤轻重不一 。

Objective To explore the effects of intestinal trefoil factor (ITF) on interleukin-8(IL-8) and malondiadehyde (MDA) of neonatalrats' intestinal tissue post necrotizing enterocolitis(NEC), to investigate whether ITF has some protective effects on NEC.. Methods Thirty-two neonatal tats of NEC model were devided into four groups, (1)NEC+FTF 0.5 mg, (2) NEC+ITF 0.2 mg, (3) NEC, and (4) all others. After NEC model of neonatal rats was established and the ITF was treated, all the neonatal rats were returned to their mothers. In the 4th day, all the subjects were put to death. Intestinal tissue located at the boundary of ileum and cecum was obtained to observe hostological changes. Other intestinal sissue was removed to homogenate. Than the homogenate was centrifuged, supernates were used to determine the content of IL-8 and MDA. Results Content of IL-8 was dramatically less in group A and B than group C, respectively (29.722±7.134)、(30.512± 8.230)、(39.379 5±4.420) [pg/(mg·pro)](P<0.05), but no differences were found between A, B and D (23.922±6.168) (P>0.05). Content of MDA was dramatically less in group A and B than group C, respectively(2.267±0.267)、(2.154±0.301)、(3.378±0.835)[nmol/(mg·pro)](P< 0.01), No differences were found between A, B and D(2.225±0.443)(P<0.01).In group C,the histological lesions ranged from superficial epithelial damage with villous shortening to transmural necrosis and was graded 3 point. Only some villous epithelial necrosis and vascular congestion was seen in group A and B, and was graded 1 point. Conclusions Intestinal inflammation was ameliorated after ITF was injected hypodermically or intraperitoneally. ITF may provide a brand-new way for the therapy of NEC in neonatal rats.