甲硝唑对地塞米松预处理鸡免疫功能的影响

In vivo effects of metronidazole on the immunity function of dexamethasone-treated chicks

对地塞米松预处理鸡投以10mg/kg剂量的甲硝唑,观察后者对前者作用的影响。结果表明:地塞米松明显抑制由伴刀豆球蛋白A(ConA)引起的鸡外周血淋巴细胞(PBL)产生IL-2活性,而甲硝唑对这种抑制作用有显著的拮抗作用;地塞米松能抑制鸡对绵羊红细胞(SRBC)悬液和牛血清白蛋白(BSA)两种胸腺依赖性抗原的免疫应答和对布鲁氏菌非胸腺依赖性抗原的抗体应答,但甲硝唑会逆转这种抑制作用;甲硝唑能封闭地塞米松对补体产生的抑制作用。对比实验结果同时表明,在拮抗地塞米松对鸡免疫功能负面影响方面,左旋咪唑与甲硝唑作用非常类似。

The effects of metronidazole in vivo were studied on the ConA and LPS response of chick peripheral blood lymphocyte(PBL), on the IL2 activity of PBL induced by ConA, on the antibody response to two thymus dependent antigens SRBC and BSA, and to a thymus independent antigen Brucella abortus(BA) and on theproductionof complement of dexamethasonetreated chicks. The results indicated that dexamethasone treatment had no effects on the ConA and LPS response of chick PBL. It was shown that metronidazole at dose of 10 mg/kg could significantly antagonize the inhibition of dexamethasone on the IL2 activity of PBL induced by ConA, reverse the suppression of dexamethasone on antibody responses to SRBC, BSA, and BA, block the negative effects of dexamethasone on the production of complement. Levamisole had very similar effects like metronidazole in antagonizing the negative effects of dexamethasone on the immune function of chicks. It was observed that levamisole at a dose of 10 mg/kg could significantly antagonize the inhibition of dexamethasone on the IL2 activity of PBL, on antibody responses to SRBC, BSA and BA, and on the production of complement,respectively.