瘦素及其受体系统在女性生殖器官的表达

Expression of leptin and leptin receptor system in woman reproductive organs

目的 研究瘦素 (leptin)及其受体 (Ob R)、瘦素信号传导相关蛋白质在女性生殖器官的表达 ,探讨瘦素对女性生殖系统的影响。方法 分别用免疫组织化学和原位杂交技术检测瘦素、具有信号传导功能的瘦素长受体 (Ob Rb)、磷酸化的细胞因子信号传导转录活化因子STAT3(p STAT3)、细胞因子信号传导抑制剂 3(SOCS3)、活化的STAT3蛋白质抑制剂 (PIAS3)在正常妇女子宫内膜 ( 39例 ,增殖期 2 4例 ,分泌期 15例 )、子宫颈和阴道及输卵管 ( 3例 )的表达。结果 ( 1)瘦素、Ob RbmRNA及其蛋白质在子宫内膜的腺体及间质均呈阳性表达。 ( 2 )Ob Rb蛋白质在分泌期子宫内膜的表达 3例弱阳性 ,3例阳性 ,9例强阳性 ;在增殖期子宫内膜的表达为 2例阴性 ,19例弱阳性 ,3例阳性 ,两者比较差异有显著意义 (P <0 .0 1)。瘦素蛋白质在不同期子宫内膜的表达差异无显著意义。 ( 3)瘦素及受体蛋白质在子宫颈、阴道、输卵管的上皮 (包括腺体 )均有表达。 ( 4)信号传导相关蛋白质SOCS3、PIAS3在子宫内膜和子宫颈的腺上皮、输卵管的纤毛上皮呈阳性表达 ,p STAT3表达极弱或不表达。结论 瘦素可能通过受体系统 ,与生殖器官间有广泛联系 ,影响女性的生殖功能。

Objective To explore the expression of leptin, leptin receptor (Ob-R) and related signaling proteins in woman reproductive organs. Methods Thirty-nine women, aged 27～37, with normal menstrual cycle of which 24 being in proliferative phase and 15 in secretive phase, underwent uterine curettage, to obtain the endometrium. The uterine cervix, vaginal wall, and oviducts were resected from 3 women with hysteromyoma. The expression of mRNA of leptin and long isoform of leptin receptor (Ob-Rb) was detected by in situ hybridization. The expression of leptin and Ob-Rb proteins was examined by immunohistochemical staining. Immunohistochemical staining was used to study the expression of the leptin-related signaling proteins: phosphated signal transducers and activator of transcription (p- STAT3), suppressor of cytokine signaling 3 (SOCS3), and protein inhibitor of activated STAT3 (PIAS3). Results The mRNA and protein of leptin and the mRNA and protein of Ob-Rb were expressed in the glands and mesenchyma of endometrium. The expression of Ob-Rb protein was significantly stronger in secretive phase than in proliferative phase (P<0.01). No significant difference was found between the expression of leptin protein in proliferative phase and that in secretive phase. In the endothelium SOCS3 and PIAS3 were expressed, however, p-STAT3 protein was only weakly expressed or not expressed. Leptin protein and Ob-Rb protein were expressed in the uterine cervix, vagina wall, and epithelium of oviduct, including glands. However, leptin mRNA and Ob-Rb mRNA were only expressed in the ciliated epithelium of oviduct. Conclusion Leptin may have an effect on women′s reproductive function through leptin receptor systems.