脱氢表雄酮增强老龄大鼠免疫功能和记忆力的研究

Enhancement Roles of Dehydroepiandrosterone on Immune Function and Memory Abilities in Older Rats

目的 了解脱氢表雄酮 (DHEA)对老龄大鼠体重、记忆和免疫功能的影响。方法 采用灌胃法 ,把 DHEA溶液从食道中灌入 ,然后采用一次性避暗学习 ,检测大鼠的记忆情况 ;用 3H掺入法测定淋巴细胞的转化 ;以 YAC- I细胞株为靶细胞 ,测定自然杀伤细胞 (NK)的活性 ;用标准酶联免疫吸附法 (ELSA)测定大鼠血清中 Ig G水平 ;并从给药的当天开始 ,测定大鼠体重 ,每隔 4d1次。结果 喂食 DHEA后老龄大鼠的细胞免疫和自然杀伤细胞活性都增强 ;记忆的保持能力也有所提高 ,与对照组相比达到显著性 ,接近青龄鼠的水平。另外 ,DHEA对老龄鼠体重的减轻也有显著作用 ,而对青龄鼠则不明显。结论 DHEA具有一定的抗衰老作用。

Objective To study the effect of dehydroepiandrosterone (DHEA) on the weight, memory and immune function in older rats.Methods The DHEA was filled into the esophagus of the experimental group of rats for forty days (once each other day). Then the memory abilities were measured by one-trial passive avoidance task and the T and B-lymphocyte transformations were examined by 3H-TdR incorporation experiment. The enzyme linkedimmunosorbant assay (ELISA) was used to determine the plasma IgG level in rats.The cell line YAC-I was used as targets to determine the NK activity and the weight of rats was weighed on the experimental day (once each three days).Results After DHEA treatment, the cell-mediated immunity, humoral immunity and NK activity and the memory retention in older rats were significantly enhanced nearing the level of young group compared to the control group. In addition, the DHEA might reduce the weight of the older rats markedly,but this effect to the young rats was not distinct.Conclusions The DHEA has an antiaging actions.