抗人膀胱癌/抗血管内皮生长因子双功能基因抗体的动物实验研究

Animal experiment of bispecific antibody(BsAb,anti-human bladder cancer/anti-VEGF) on human bladder cancer growth

目的 探讨抗人膀胱癌 /抗人血管内皮生长因子 (VEGF)双功能基因抗体对膀胱癌生长的抑制作用。方法 应用双功能基因抗体作用于荷瘤动物模型观察抑瘤作用 ,应用免疫组化技术检测体内生长肿瘤中微血管密度。结果 双功能基因抗体对体内生长的肿瘤有明显的抑制作用 ,经双功能基因抗体治疗的瘤体中微血管密度明显低于对照组。结论 双功能基因抗体能够抑制肿瘤血管生成 ,从而为膀胱癌的治疗提供一种新的辅助方法。

Objective To study the inhibitory role of BsAb(anti-human bladder cancer/anti-VEGF) on human bladder cancer growth.Methods BsAb was used to treat the tumor models of BIU-87 cell lines.The tumor inhibition rate was calculated and the tumor microvessel density was detected by immunohistochemical techniques.Results BsAb could significantly inhibit the growth of tumor.And the tumor microvessel density was lower than that of the control group.Conclusions BsAb could inhibit the tumor growth by inhibiting angiogenesis. BsAb may become a new method for human bladder cancer treatment.