应用氯化锶对小鼠卵母细胞孤雌活化的研究

Study on the Induction of Parthenogenetic Activation in Mouse Oocytes by Strontium Chloride

目的 探讨小鼠卵母细胞孤雌激活的最佳作用条件。方法 将MⅡ期小鼠卵母细胞随机分为 2组 ,第一组 ,将小鼠卵母细胞分别放入 2、4、6、8、10mmol ml不同浓度的氯化锶激活液中 ,作用 6h ,观察激活率及囊胚发育率。第二组 ,将小鼠卵母细胞放入 10mmol ml氯化锶激活液中 ,分别作用 3、6、9h ,观察激活率及囊胚发育率。结果 第一组 ,激活率分别为 86 49%、82 6 1%、88 0 0 %、86 6 7%、81 18%。各组间差异无显著性。体内培养 72h回收囊胚 ,囊胚发育率分别为 0、31 42 %、43 33%、6 2 5 0 %、5 0 0 0 %。 6～ 10mmol mlSrCl2 激活液激活后囊胚发育率高于 0～ 4mmol ml(P <0 0 5 )。第二组 ,激活率分别为 82 86 %、89 6 1%、91.40 %。 72h囊胚发育率分别为 2 6 5 3 %、5 0 0 0 %、5 3 2 2 %。激活 6、9h的囊胚发育率高于激活 3h的囊胚发育率 (P <0 0 1)。结论 结果表明 ,6～ 10mmol ml的SrCl2 为卵母细胞孤雌活化的最佳作用浓度 ,6～ 9h的激活时间为最佳作用时间 ;表明SrCl2 的浓度和作用时间对小鼠卵母细胞的活化有显著的影响。

Objective The experiment was conducted to study the effects of the optimum condition of strontium chloride on the parthenogenesis of mouse oocytes.Methods Experiment Ⅰ: The MⅡoocytes of mouse were activated with 0, 2, 4, 6, 8, 10mmol/ml SrCl 2 respectively for 6 h and the activation rate and blastocyst development rate observed. Experiment Ⅱ: MII oocytes were activated with 10 mmol/ml SrCl 2 for 3, 6, 9 h respectively, the activation rate and blastocyst development rate observed.Results Experiment Ⅰ:activation rates of oocytes in different concentration of SrCl 2 were not significant difference(86.49%,82.61%, 88.00%, 86.67%, 81.18% respectively), and blastocyst development rates were 0, 31.42%, 43.33%, 62.50%,50.00% respectively. Blastocyst development rates when oocytes treated with 6-10 mmol/L SrCl 2 were significantly higher than those treated with 0-4 mmol/ml SrCl 2.Experiment Ⅱ: activation rates were 82.86%, 89.61%, 91.40% respectively. Blastocyst development rates were 26.53%, 50.00%, 53.22% respectively. Blastocyst development rates when oocytes treated with 10 mM SrCl 2 for 6, 9 h were significantly higher than those when the oocytes were treated with the same medium for 3 h (P<0.01). Conclusion 6-10 mmol/ml SrCl 2 were the optimum concentration for induction of parthenogenetic activation in mouse oocytes. 6-9 h was the best time for activation. It is showed that the SrCl 2 concentration and the duration subjected to SrCl 2 have great effect on mouse oocytes activation and early parthenogenetic development.