剑尾鱼近交系遗传纯度的RAPD分析

Analysis of Genetic Purity of Swordtail Fish Inbred Strain by RAPD

目的 分析近交系剑尾鱼 (Xiphophorushelleri)的遗传纯度 ,以指导剑尾鱼实验动物化的培育工作。方法 应用经过筛选的 2 9条随机引物对剑尾鱼的第 19代近交系 12尾剑尾鱼个体基因组DNA进行RAPD扩增 ,计算近交系个体间的相似系数及遗传距离。结果 筛选的 2 9条引物共扩增出 30 6条带 ,扩增片段的大小在 0 2 - 3kb之间。其中多态性带 2 9条 ,多态性带频率在 0～ 5 0 0 0 %之间 ,平均为 9 48%。近交系剑尾鱼不同个体拥有相同条带的比率较高。计算得到近交系的平均相似系数 (S)为 0 9839(0 973～ 0 997) ,平均等位基因频率 ( q)为 0 8731,最低平均杂合率 ( H)为 0 12 6 9。 结论 剑尾鱼第 19代近交系有较高的遗传纯合度。

Objective To analyze the genetic purity of swordtail fish (Xiphophorus helleri) inbred strain for directing the breeding of swordtail as laboratory animal. Methods 29 selected single primers were used to amplify randomly genomic DNA of the inbred strain of swordtail fish. The amplified fragment bands were analyzed to calculate the similarity index and the genetic distance. Results 306 bands with the lengths between 200bp and 3kb were obtained, among them there were 29 polymorphic bands, the frequency of polymorphic bands was between 0-50.00% and the average was 9.48%. There was a high frequency of common band in different individuals. Based on RAPD patterns, the calculated similarity index within 12 samples of the inbred strain was 0.9839(0.973-0.997), the mean allelic frequency was 0.8731, and the minimum heterozygosites was 0.1269. Conclusion The inbred strain of swordtail fish has reached a high degree of genetic purity.