灵光注射液对失血性休克大鼠心脏和肝脏的影响

The Effect of Compound Anisodine on Heart and Liver after Hemorrhagic Shock in Rats

目的 观察灵光注射液 (复方樟柳碱 )对失血性休克再灌注大鼠心脏和肝脏损伤的影响。方法 将5 6只雄性大鼠随机分 4组 ,分别设为假休克组 (8只 )、模型组 (16只 )、灵光注射液低剂量组 (16只 )和高剂量组 (16只 ) ,除假休克组外 ,大鼠均经历 4kPa ,70min的失血性休克 ,在休克复苏后 6h和 12h各组分别处死半数动物 ,检测血清CK、CK MB、LDH、ALT、AST ,心脏和肝脏做组织学和超微结构检查。结果与结论 灵光注射液对大鼠失血性休克再灌注引起的心脏和肝脏功能和形态损伤均有明显的治疗作用 ,其机制可能与改善微循环、清除氧自由基和保护生物膜作用有关。

Objective To observe the effect of LINGGUANG injection(compound anisodine)on heart and liver after hemorrhagic shock in rats. Method Fifty-six male Wistar rats were divided into 4 groups randomly, which were sham shock, shock, shock treated with LINGGUANG injection in low dose and high dose groups separately. Animals were subjected to sham shock, or 70 minutes shock at 4-kPa blood pressure with or without LINGGUANG injection treatment. Half of the rats were sacrificed at 6 hours after resuscitation, the rest were killed at 12 hours. The CK,CK-MB,LDH,ALT,AST of sera were detected. The morphological changes of hearts and livers were observed under light microscope and transmission electron microscope. Results and Conclusion Compound anisodine is effective for curing the damages of heart and liver caused by hemorrhagic shock and reperfusion. The mechanism of compound anisodine treatment may be related with microcirculation improving, free radical scavenging.