HBME-1在恶性上皮性间皮瘤的表达及其意义

EXPRESSION AND SIGNIFICANCE OF HBME-1 IN MALIGNATBNT EPITHELIAL MESOTHELIOMA

目的 通过研究HBME 1在恶性上皮性间皮瘤 (MEM )的表达及其与DNA含量的关系 ,探讨HBME 1在MEM与转移性卵巢腺癌 (OA)和转移性结直肠腺癌 (CA)的鉴别诊断中的意义。方法 采用免疫组化SP法染色 ,观察MEM、OA和CA组织标本中HBME 1的表达 ;同时利用图象分析仪对MEM的细胞DNA含量进行定量分析。结果 HBME 1的表达在MEM与CA两组间的差异有显著性 (P <0 0 1) ,在MEM与OA两组间的差异无显著性 (P <0 0 5 ) ,但着色方式不同 ;在MEM中 ,HBME 1表达阳性组与阴性组之间的DNA含量差异无显著性 (P <0 0 5 )。结论 HBME 1的表达与否与MEM的DNA含量无关 ,HBME 1可作为MEM与CA鉴别的阳性标记物的参考指标 ,在MEM与OA的鉴别中也有一定参考价值。

? Objective To study the relationship between the expression of HBME-1 and the DNA content in malignant epithelial mesothelioma (MEM), in order to investigate the role of HBME-1 in distinguishing MEM from metastatic ovarian adenocarcinoma (OA) and colorectal adenocarcinoma (CA). Method Immunohistochemical SP method was used to detect the expression of HBME-1 in paraffin-embedded tissues from 18 MEM、20 OA and 20 CA cases. Image analysis was used to measure the cell DNA content of the 18 MEM cases. Result There was a significant difference in the expression of HBME-1 between MEM and OA groups(P<0.01), but not between MEM and OA groups (P>0.05). There was no significant difference in the cell DNA content between the HBME-1 positive and negative MEM groups (P>0.05). Conclusion The expression of HBME-1 does not correlate with the cell DNA content in MEM, and HBME-1 could be used as a positive marker in distinguishing MEM from CA. In consideration of the characteristic staining pattern of HBME-1 in MEM, it may serve as an index to discriminate MEM from OA. 〔