恶性肿瘤转移与患者免疫活性细胞水平的相关性研究

Relativity study between tumor metastasis and the level of immune competent cells in patients with malignant carcinoma

目的 :探讨肿瘤转移和患者机体免疫活性细胞检出率的关系。方法 :用流式细胞术对恶性肿瘤患者肿瘤组织和外周血的自然杀伤细胞、细胞毒T细胞和单核 巨噬细胞的检出率进行了检测和分析。结果 :恶性肿瘤患者的组织和血液中的自然杀伤细胞、细胞毒T细胞和单核 巨噬细胞的检出率均显著低于相应的对照组 (P <0 0 1或P <0 0 5 )。恶性肿瘤转移者的自然杀伤细胞、细胞毒T细胞和单核 巨噬细胞检出率均显著低于未转移者 (P <0 0 1或 P <0 0 5 )。肿瘤组织和外周血的自然杀伤细胞、细胞毒T细胞和单核 巨噬细胞的检出率在各种情况下均呈现平行性的变化。结论 :恶性肿瘤能引起患者机体免疫活性细胞的水平显著降低 ,肿瘤转移使患者机体免疫活性细胞水平进一步下降。通过对恶性肿瘤患者外周血免疫活性细胞检测可以了解肿瘤组织免疫活性细胞的变化趋势。

Objective:To study relativity between tumor metastasis and the level of immune competent cells from patients with malignant carcinoma.Methods:The percentage of NK cells and CTL cells and monocyte/macrophage cells were measured and analyzed in tumor tissue and peripheral blood from patients with malignant carcinoma by flow cytometry.Results:NK cells, CTL cells and monocyte/macrophage cells of tumor tissue and peripheral blood were lower than those of normal control groups ( P <0.01 or P <0.05). The percentage of immune competent cells in tumor tissue and peripheral blood from patients with tumor metastasis were lower than those from patients with non-matestasis ( P <0.01 or P <0.05).The expression level of immune competent cells between tumor tissue and peripheral blood were showed parallel changes at different conditions.Conclusion:Malignant tumor may lead to significant decrease of the level of immune competent cells from patients with malignant carcinoma. Tumor metastasis may lead to progressively decrease of the level of the immune competent cells from patients with malignant carcinoma. In addition, the measurement of the level of immune competent cells in peripheral blood may comprehend the change tendency of immune competent cells of tumor tissue from patients with malignant carcinoma.