乳胶致小鼠气道炎症的研究

Study on the airway inflammation of mouse model induced by latex

目的 :以乳胶为致敏原 ,建立具有哮喘主要特征的小鼠模型 ,研究乳胶与气道炎症之间的关系。方法 :乳胶腹腔注射与滴鼻诱发BALB C小鼠气道炎症 ,在激发后 2 4小时行支气管肺泡灌洗液 (BALF)细胞计数分类、肺组织病理检查、血清总IgE及乳胶特异性IgE(sIgE)测定并检测IL 5、IFN γ的表达。结果 :乳胶诱发后 ,实验组小鼠BALF中细胞总数及嗜酸粒细胞百分比升高 ;肺组织病理切片示支气管上皮增生、脱落 ,粘液腺分泌现象 ,支气管痉挛、收缩 ,炎症细胞浸润 ;血清总IgE及乳胶sIgE水平升高 ;BALF及肺组织局部IFN γ减少 ,IL 5增高。结论 :用乳胶蛋白作为致敏原 ,采用腹腔致敏与多次滴鼻激发可使BALB C小鼠发生气道炎症 ,具有与变应原诱导的人类哮喘迟发反应相一致的主要特征 :气道变应性炎症 ;外周血IgE浓度升高 ;肺组织中Th2型CK表达占优势。

Objective:To establish a mouse model with the main characteristics of asthma and to study the pathogenesis of its airway inflammation.Methods:Mice were given latex by the intraperitoneal sensitization/intranasal challenge.BALF histology were examined 24 hours following tracheal challenge;cytokines and serum total and specific IgE were examined in one month and the expression of IL-5?IFN-γ were performed by ELISA ?RT-PCR.Results:After the latex challenge,in experimental group,the number of total BALF cells and the percentage of eosinophils elevated;the histological analysis showed the elevation of mucus production and goblet cell development within bronchial epithelium,spasm and constraction of bronchia ,marked peribronchial infiltration of eosinophils and lymphocytes; the elevation of total and specific IgE;reduction of IFN-γ and increase of IL-5 in the BALF and lung.Conclusion:Latex sensitization and challenge can lead to airway eosinophilia,allergen-specific IgE induction and production of Th2 type cytokines, which were the main characteristics of asthma.