幽门螺杆菌对胃癌及癌旁组织中Bcl-2家族基因表达的影响

EFFECT OF HELICOBACTER PYLORI INFECTION ON GENE EXPRESSIONS OF Bcl-2 FAMILY MEMBERS IN GASTRIC ADENOCARCINOMA AND TISSUE ADJACENT TO THE TUMOR

目的 研究幽门螺杆菌 (H .pylori)感染对胃癌及癌旁组织中Bcl 2家族基因表达的影响。方法 收集 95例胃癌及癌旁组织标本 ,应用半定量RT PCR法检测Bid、Bax和Bcl 2mRNA的表达。结果 胃癌组织中Bid和BaxmRNA表达按H .pylori阴性组、Ca gA-H .pylori感染组和CagA+ H .pylori感染组顺序依次增高 (P <0 0 5 ) ,H .pylori阴性组的Bcl 2mRNA表达低于H .pylori阳性组 (P<0 0 5 ) ;癌旁组织中 ,Bid、Bax和Bcl 2表达按H .pylori阴性组、CagA-H .pylori感染组和CagA+ H .pylori感染组顺序均依次递增 (P <0 0 5 )。H .pylori阴性组中 ,胃癌组织的Bid和Bax表达与Bcl 2表达呈负相关 (P <0 0 5 ) ,而在H .pylori感染组中 ,胃癌组织的Bid和Bax表达与Bcl 2表达无相关性 ;癌旁组织的Bid和Bax表达与Bcl 2表达呈正相关 (P <0 0 5 )。结论 H .pylori有促进Bid、Bax和Bcl 2表达的作用 ,并且能够诱发这些细胞凋亡调控基因的表达紊乱 ,这也许是H .pylori诱导胃癌发生的途径之一。

Objective To investigate the effect of Helicobacter pylori ( H.pylori ) infection on the expressions of Bcl 2 family members in gastric adenocarcinoma was investigated. Methods Gastric adenocarcinoma tissue and tissue adjacent to it obtained from 95 patients were collected. Semi quantitative RT PCR was used to measure Bid, Bax and Bcl 2 mRNA levels. Results Expressions of Bid and Bax were increased significantly in the following order: gastric adenocarcinoma tissue without H.pylori infection, that with cagA - H.pylori infection, and that with cagA + H.pylori infection (Bid, 0 304, 0 422 and 0 855 respectively, P <0 05; Bax, 0 309, 0 650 and 0 979 respectively, P <0 05). Bcl 2 mRNA levels in gastric adenocarcinoma tissue with cagA - H.pylori and cagA + H.pylori infection were significantly increased compared with those without H.pylori infection (0 696 and 0 849 vs 0 411, P <0 05). In tissues adjacent to the tumor, the expressions of Bid, Bax and Bcl 2 were shown to be increased in the following order: no H.pylori infection <cagA - H.pylori infection <cagA + H.pylori infection (Bid, 0 377, 0 686 and 0 939 respectively, P <0 05; Bax, 0 353, 0 645 and 1 001 respectively, P <0 05; Bcl 2, 0 371, 0 487 and 0 619 respectively, P <0 05). In H.pylori negative specimens, expressions of Bid and Bax correlated negatively with that of Bcl 2 in gastric adenocarcinoma tissues (Bid vs Bcl 2, r =-0 409, P <0 05; Bax vs Bcl 2, r =-0 451, P <0 05). In H.pylori positive specimens, expressions of Bid and Bax did not correlate with that of Bcl 2 in adenocarcinoma tissues (Bid vs Bcl 2, r =0 187, P >0 05; Bax vs Bcl 2, r =0 201, P >0 05), but correlated positively with that of Bcl 2 in adjacent gastric tissue (Bid vs Bcl 2, r =0 331, P <0 05; Bax vs Bcl 2, r =0 295, P <0 05). Conclusion H.pylori may enhance Bid, Bax and Bcl 2 mRNA levels and cause disturbance in regulation of expression of these apoptosis associated genes, which may play a role during the development of gastric adenocarcinoma induced by H.pylori .