白介素8和干扰素α对离体培养的子宫内膜细胞的影响

EFFECTS OF IL-8 AND IFN-α ON CULTURED ECTOPIC ENDOMETRIAL CELLS

目的 探讨白介素 8(IL 8)和干扰素α(IFN α)对体外培养的子宫内膜异位症患者的子宫内膜细胞的影响。方法 收集患者的子宫内膜组织进行体外培养、纯化 ,采用免疫组织化学的方法鉴定纯化的细胞。在培养基中加入不同浓度的IL 8(0 1、1、5、10、5 0ng/ml)和IFN α 2b(10、5 0、10 0、10 0 0、10 0 0 0U/ml) ,对腺上皮细胞进行培养 ,2 4h后用MTT法检测细胞增殖的状况。同时 ,用含有 1ng/mlIL 8的培养基对内膜细胞进行培养 ,2 4h后用透射电镜观察IL 8对腺上皮细胞和间质细胞的影响。结果 细胞的增殖与IL 8的含量有着明显的剂量依赖关系 ,并且在IL 8浓度为 10ng/ml时最为显著(P <0 0 5 )。电镜观察发现在 1ng/ml的IL 8的作用下 ,内膜细胞功能活跃 ,分泌旺盛 ,可见细胞外有胶原产生。而IFN α 2b对腺上皮细胞的增殖却有明显的抑制作用 (P <0 0 5 )。结论 本研究明确了IL 8和IFN α 2b对内膜细胞的影响 ,并且发现IL 8可以使内膜细胞分泌胶原纤维增多 ,这可能在子宫内膜异位症的发病机制中起着重要作用。IL 8可能是通过自分泌或者旁分泌的方式参与发病的。IFN α 2b是一类免疫正调因子 ,它可以调节子宫内膜异位症患者的免疫功能。免疫刺激疗法有可能成为治疗子宫内膜异位症的一种新的方法。

Objective The purpose of this study was to investigate the effects of interleukin 8 (IL 8) and interferon (IFN) alpha on proliferation of ectopic endometrial cells in vitro . Methods Ectopic endometrial cells from women suffering from endometriosis were cultured, purified, and identified by immunocytochemistry. Cultured endometrial cells were treated with IL 8 and IFN α 2b in various concentrations in 96 well plates (IL 8 in 0 1ng/ml, 1ng/ml, 5ng/ml, 10ng/ml, 50ng/ml, IFN α in 10U/ml, 50U/ml, 100U/ml, 1 000U/ml, 10 000U/ml). The effects of IL 8 and IFN α 2b on endometrial cell proliferation were assessed using MTT. The ultrastructural changes in endometrial cells, which were incubated in medium containing 1ng/ml of IL 8, were observed with transmission electron microscopy. Results The data suggested that there was a dose dependent stimulatory effect of IL 8 on cell proliferation. IL 8 could stimulate proliferation of endometrial cells, and the most effective concentration was 10ng/ml ( P <0 05). At the concentration of 1ng/ml IL 8, endometrial cells showed a high secretory activity and produced collagen at 24 hours of culture. On the other hand, IFN α 2b at a concentration of 10 000U/ml could significantly inhibit the proliferation of endometrial cells ( P <0 05). Conclusions Our findings confirmed the effects of IL 8 and IFN α 2b on endometrial cells, and indicated that IL 8 could induce collagen secretion from endometrial cells, which might play an important role in the pathogenesis of endometriosis. IL 8 might participate in the pathogenesis of endometriosis by autocrine or paracrine. IFN α 2b was a positive immuno modulatory factor, and it could modify the immune system in patients with endometriosis. Imunostimulative therapy may be a potential new method for the treatment of endometriosis