摘要
Microporous polyamide-6 membranes were hydrolyzed in 0.01 mol/L NaOH/CH 3OH medium by refluxing for about 36 h. By investigating the UV spectra, the hydrolyzing conditions were optimized. A pH value of 12 and reaction time of 36 h are the preferred conditions for hydrolysis. The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P 2p. The single step coupling efficiency was determined by UV spectra to be in excess of 98 %.
Microporous polyamide-6 membranes were hydrolyzed in 0.01 mol/L NaOH/CH 3OH medium by refluxing for about 36 h. By investigating the UV spectra, the hydrolyzing conditions were optimized. A pH value of 12 and reaction time of 36 h are the preferred conditions for hydrolysis. The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P 2p. The single step coupling efficiency was determined by UV spectra to be in excess of 98 %.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第11期2089-2091,共3页
Chemical Journal of Chinese Universities
基金
国家"八六三"计划 (批准号 :10 1-0 1-0 1)
"九七三"计划 (批准号 :G19980 5 12 0 4)
国家自然科学基金 (批准号 :60 0 710 0 1)
江苏省高新技术项目 (批准号 :BG2 0 0 10 10 )
教育部高等学校骨干教师资助计划基金资助
