野生马氏珠母贝子一代的遗传多样性

Genetic Diversity of Two Wild Pinctata martensi Populations′ F1 Generations

分析了海南省三亚与广西省北海两地野生贝群体内交配所得子代两群体 (SS、BB)和群体间交配(SS♀ ×BB♂ →BS)所得子代群体 (BS)各 8个个体的遗传多样性。筛选的 2 0个含 1 0碱基的随机引物中 ,其中 1 4个产生稳定的可重复的多态扩增结果 ,共检测出 1 2 4个位点。用修正的Shannon表型多态性指数量化三个群体的遗传多态度 ,SS、BB、BS三群体的多态度分别为 0 2 47,0 2 3 7,0 2 61。SS与BB ,SS与BS ,BB与BS三群体间的遗传相似度分别为 87 2 4% ,80 3 9% ,83 90 %。

Pinctata martensi is one of the most important seashells in the southeast of China. In order to improve our understanding of this species and the efficiency of breeding by marker\|assisted selection, we used 20 primers to test different populations with the RAPD technique. We randomly collected wild individuals in Sanya (SS), Hainan Province and Beihai (BB), Guangxi Province. We allowed both intra and inter\|population mating (SS\-♀×BB\-\{♂\}→BS) among these individuals, getting three filial populations known as SS,BB and SB. Among the 20 primers, 14 produced highly reproducible profiles and generated 124 bands among the three filial populations. The genetic diversity index of the three populations (SS, BB and SB) was 0 247, 0 237 and 0 261 respectively. Genetic similarity between the two populations (SS with BB, SS with SB, BB with SB) was 87 24%,80 39%,83 90%. Finally, we analyzed the feasibility of interbreeding individuals from these two different areas.