摘要
目的评价人参炔醇对代谢障碍、兴奋性氨基酸、氧自由基三种脑片损伤模型的作用。 方法通过大鼠皮质和海马脑片孵育技术,建立缺氧缺糖、谷氨酸和过氧化氢三类损伤模型,采用新鲜脑片TTC染色酶标仪比色法,定量考察人参炔醇对不同类损伤的保护作用。 结果与对照组相比,缺氧缺糖、谷氨酸和过氧化氢均能明显降低皮质和海马脑片TTC染色OD490值,2～4μmol/L人参炔醇可显著减轻缺氧缺糖及过氧化氢所致的脑片组织损伤(P<0.01),对谷氨酸所致损伤无作用。3μmoL/L cAMP阻断剂Rp cAMPS可显著减弱4μmol/L人参炔醇对缺氧缺糖和过氧化氢所致损伤的保护作用。 结论人参炔醇能够保护大鼠脑片对缺氧缺糖和过氧化氢所致损伤作用,但对谷氨酸所致损伤无保护作用,其保护活性可能与提高神经细胞内cAMP含量有关。
Objective To investigate the effect of panaxynol on the different injury models of brain slices. Methods The injury models of rat cortical or hippocampal slices were induced by oxygen and glucose deprivation (OGD) , glutamine or hydrogen peroxide ( H2O2 ) respectively. TTC assay was used to evaluate tissue activity. Results TTC staining ( OD490 ) of cortical or hippocampal slices in the injury group decreased significantly compared with control group. 2 - 4μmol/L of panaxynol could significantly attenuate tissue injury induced by OGD and H2O2(P <0. 01) . Panaxynol showed no protective effect on the injury model induced by glutamine. 3μmol/L of RpcAMPS could offset the protective effect of panaxynol (4@@mol/L) from brain slice injuries induced by OGD and H2O2 Conclusion Panaxynol could protect brain slice injuries induced by OGD and H2O2 dose - dependently, which may be related with the persistent increase of cAMP in neuron.
出处
《上海第二医科大学学报》
CSCD
2003年第6期485-488,507,共5页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市教委学科建设专项基金资助(JY2001)
上海交通大学-上海第二医科大学联合科研基金资助(2003HZJJ002)
