摘要
目的探讨氟尿嘧啶耐药的人胃癌细胞裸鼠移植瘤血管生成与肿瘤耐药产生机制的关系。 方法建立裸鼠移植瘤模型;逆转录多聚酶链反应(RT—PCR)法检测血管内皮生长因子(VEGF)mRNA的表达;免疫组织化学方法检测裸鼠移植瘤VEGF蛋白的表达;免疫组织化学方法进行血管内皮细胞染色,计算肿瘤组织内微血管密度。 结果成功建立耐药和亲本两组裸鼠移植瘤。与其亲本细胞裸鼠移植瘤比较,耐药细胞裸鼠移植瘤组织VEGFmRNA和蛋白质表达以及微血管密度都显著减少(P<0.05)。 结论胃癌的耐药机制可能与血管生成减少有关。
Objective To investigate the relationship of angiogenensis and the mechanisms of drug resistance in human gastric drug resistant cancer xenograft of nude mice. Methods Establishing cancer xenograft model in nude mice. The mRNA expression for vascular endothelial growth factor ( VEGF) was measured by reverse transcription polymerase chain reaction ( RT - PCR). The protein expression of VEGF in the xenograft of nude mice was determined by immunohistochemistry. Vascular endothelial cells were stained by immunohistochemistry and intratumoral microvessel density (MVD) was counted. Results The two teams of cancer xenograft of nude mice, the drug resistant and the parent, were successfully established. Comparing parent cancer xenograft, the mRNA expression and protein expression of VEGF and microvessel density of the drug resistant cancer xenograft were significantly decreased(P <0. 05) . Conclusion The mechanism of drug resistance of gastric cancer may be related with the decreased angiogenesis.
出处
《上海第二医科大学学报》
CSCD
2003年第6期495-497,514,共4页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市科技发展基金资助项目(004119048)
