几种趋化因子在伴放线放线杆菌刺激的巨噬细胞中的表达

Study on the Expression of Chemokines in Macrophage Stimulated by Actiniobacillus Actinomycetemcomitans

目的探讨趋化因子在伴放线放线杆菌刺激的巨噬细胞中的表达情况。 方法将伴放线放线杆菌和巨噬细胞共同培养,利用β-actin作为内参照,半定量逆转录PCR(RT—PCR)法,测定在不同时间点巨噬细胞表达趋化因子(IL-8、MIP-1α、RANTES、MGSA、MIG)mRNA的相对量。 结果共同培养6h后,巨噬细胞中IL-8和MIP-1α mRNA表达量明显增加(P<0.05,P<0.01),并且随着培养时间的延长,表达量也增加。趋化因子RANTESmRNA的表达量无明显变化。 结论趋化因子IL-8和MIP-1α mRNA表达量明显增加,提示其可能参与了以伴放线放线杆菌为优势致病菌的青少年牙周炎的免疫反应。

Objective To assay the expression of several chemokines in macrophage stimulated by actinobacillus actinomycetemcomitans( NCTC , Y4 ). Methods Actinobacillus actinomycetemcomitans and macrophage are co - cultured for several hours, then assay the quantities of each chemokines' mRNA expressed in macrophage by semi - quantify RT - PCR technique with β- actin as the reference standard. Results Co - cultured 6 hours later, the mRNA quantities of interleukin - 8 ( IL - 8 ) and macrophage inflammatory proteins - 1 alpha ( MIP - 1α) expressed in macrophage are increased ( P < 0. 05 and P < 0. 01, respectively) ,and with the co - culture continues, the expression quantites increase. The quantities of the chemokine mRNA of regulated on activation normal T cell expressed and secreted (RANTES) has no change after co -culture. The quantites of the chemokines mRNA of melanoma growth - stimulatory activity(MG-SA)and monokine induced by IFN - gamma ( MIG ) are very low and we can't detect them. Conclusion It is possibal that IL - 8 and MIP - 1α are involved in the immune reaction of juvenile periodontitis.