大鼠下丘脑及邻近区域亮-脑啡肽免疫阳性神经元与垂体后叶的关系

RELATIONSHIP BETWEEN THE LEU-ENKEPHALIN-LIKE IMMUNOREACTIVE NEURONS OF THE HYPOTHALAMUS AND ITS ADJACENT AREA AND THE POSTERIOR PITUITARY IN THE RAT

本研究用免疫细胞化学 ABC 法显示了大白鼠下丘脑内 L-ENK 免疫阳性神经元(L-ENK-ir),又进一步以垂体后叶至下丘脑 WGA-HRP 逆行追踪结合免疫细胞化学 ABC 法直接证实了下丘脑至垂体后叶的投射径路。实验结果表明:不仅下丘脑视前区,下丘脑前区及外侧区,视上核,室旁核,穹窿周核,背内侧核,腹内侧核,弓状核,乳头体前、内、外侧核及上核,终纹床核见到了 L-ENK-ir;同时在第三脑室侧壁室管膜细胞之间,穹窿下器内也见有 L-ENK 样细胞。我们分别在下丘脑视上核、室旁核、室周部、终纹床核、穹窿前核及第三脑室侧壁室管膜细胞之间见到了逆行标记 HRP 与 L-ENK-ir 的双标记细胞,说明这些部位有 L-ENK-ir 发出向垂体后叶的投射纤维。

Leu-enkphalin-like(L-ENK-L)neurons of the hypothalamus and its adjacent area of the rat were stained immunohistochemically.It has been found that besides the area previously reported in the literature L-ENK-L neurons are also present in SFO and the ependyma of the third ventricle.By combining WGA-HRP retrograde tracing and immunohistochemical method,L-ENK-L neurons in supraoptic nucleus, paraventricular nucleus,periventricular area and anterior fornical nucleus were found to project to the posterior pituitary,in which some neurons squeeze in between the ependymal cells.The subependymal neurons may serve as a link between the ven- tricle and the posterior pituitary.