芹灵冲剂对雏鸭体内DHBV-DNA的抑制作用

EFFECT OF QIN LING CHONG JI ON THE DUCK HEPATITIS B VIRUS IN VIVO

目的：观察芹灵冲剂（ＱＬ） 在鸭体内对鸭乙型肝炎病毒（ＤＨＢＶ） － ＤＮＡ 的抑制效果。方法：用ＤＨＢＶ感染雏鸭进行药物治疗试验，芹灵冲剂设３ 个剂量组，疗程１０ｄ，分离血清，样品点膜，标记ＤＨＢＶ－ ＤＮＡ探针，（ 血清） 斑点杂交，放射自显影膜片斑点检测。以杂交斑点光密度值（ＯＤ 值） 进行自身和组间比较，并作鸭肝病理检查评价。结果：芹灵冲剂高剂量组（５、１０ｇ·ｋｇ－１）能降低ＤＨＢＶ感染鸭血清ＤＨＢＶ－ ＤＮＡ 水平，给药ｄ５ 和ｄ１０ 的抑制率与病毒对照组比较，均有极显著性差异（ Ｐ ＜０ ．００１） ，并呈量效关系。低剂量组（２．５ｇ·ｋｇ－１）也有一定的疗效。肝病理镜检结果证实，空白对照组鸭肝细胞的变性坏死较重，而芹灵冲剂治疗组（ 大、中剂量） 则较轻。结论：芹灵冲剂不仅对ＤＨＢＶ有明显的抑制作用，而且有保护鸭肝细胞的作用。

AIM To study the effect of Qin Ling Chong Ji (QL) on duck hepatitis B virus (DHBV) induced hepatitis model in Nestling ducks in vivo.METHODS When positive serum DHBV was detected in seven-day infected nestling ducks,animals were randomly divided into QL 10g·kg -1 、5.0g·kg -1 、2.5g·kg -1 ,NS and positive control drug(ACV 100mg·kg -1 ) groups.Drugswere given by oral,bid for lod ten days.The blood were taken from leg vein and were separated befor treatment,after treatment on day 5 and day 10,and after stopping treatment on day 3.The presence of DHBV DNA in serum was detected by dot-blot hybridization. Probe was labelled with 32 P.RESULTS The middle and high dosage groups of QL (5 and 10g·kg -1 ) decreased the level of the infected ducks serum DHBV-DNA significantly(P<0.001), the low dosage group of QL also had the similar effect.CONCLUSION QL has inhibifory effects on and can profect duck livers from damages in hepatitis.