摘要
目的：为寻求特异性强、灵敏度高的一氧化氮测定方法，解决体内一氧化氮测定的难题。方法：用Ｎ－ 甲基－ Ｄ－葡糖胺二硫代甲酸铁［（ＭＧＤ）２Ｆｅ２＋］ 作为一氧化氮的自旋捕集剂，对小鼠肝、心、肾和肺脏中一氧化氮水平进行电子自旋共振（ＥＳＲ） 测量。结果：对１－０μｍｏｌ·Ｌ－ １的一氧化氮水平，用自旋捕集方法可得到明显的ＥＳＲ信号；对生物组织可测到１－０×１０－ ９ｍｏｌ·ｍｇ－１ 的一氧化氮。
AIM In order to find a special and sensitive method to measure NO and to solve the difficulties of probing NO in vivo.METHODS The ferrous N-methy-D-glucamine dithiocarbamate[(MGD) 2Fe 2+ ] was used as NO spin trap to probe the NO quantity in liver and heart and kidney and lung of mice.RESULTS ESR signal is obvious when NO concentration is 1 0μmol·L -1 ,the least testable quantity of NO in tissue is 1 0×10 -9 mol·mg -1 .CONCLUSION A high sensitive and distinctive method was established to measure NO in situ in biological system.
出处
《解放军药学学报》
CAS
1999年第2期12-15,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
