呋喃苯胺酸对白细胞介素-1β促骨髓基质细胞增殖的抑制作用

INHIBITORY EFFECT OF FUROSEMIDE ON PROLIFERATION OF BONE MARROW STROMAL CELLS INDUCED BY INTERLEUKIN-1β

目的：观察Ｃｌ－ 通道阻断剂呋喃苯胺酸对重组人白细胞介素－１β（ｉｎｔｅｒｌｅｕｋｉｎ－ １β，ＩＬ－ １β）诱导的小鼠骨髓基质细胞（ＢＭＳＣ） 增殖作用的影响。方法：ＭＴＴ法测定原代培养的小鼠ＢＭＳＣ增殖活性。结果：ＩＬ－１β（２００～１ ０００ｋＵ·Ｌ－１） 对ＢＭＳＣ具显著的促增殖作用。呋喃苯胺酸（２０ ～５００μｍｏｌ·Ｌ－１）特异性地抑制ＩＬ－ １β的促增殖作用，效应呈浓度依赖性。结论：Ｃｌ－ 通道与ＩＬ－ １β促ＢＭＳＣ增殖作用有关，Ｃｌ－

AIM To study the effect of chloride channel blocker furosemide on recombinant human interleukin-1β (IL-1β)-induced proliferation of mice bone marrow stromal cells (BMSC).METHODS The growth of primary cultured BMSC was determined by MTT assay.RESULTS IL-1β at the concentration of 200～1 000kU·L -1 stimulate the proliferation of BMSC significantly,and furosemide at the concentration of 20～500μmol·L -1 specially inhibited the effect of IL-1β in a dose-dependent manner.CONCLUSION Chloride channels play an important role in the signal transduction of IL-1β in bone marrow stromal cells