薄层扫描法测定马蝎蚕胶囊中士的宁和马钱子碱的含量

DETERMINITION OF STRYCHNINE AND BRUCINE IN MAXIECAN CAPSULE BY THIN LAYERCHROMATOGRAPHIC SCANNING

目的∶建立薄层扫描法测定马蝎蚕胶囊中士的宁和马钱子含量的方法。方法∶固定相 :硅胶GF2 54 ,流动相 :丙酮-氯仿 -浓氨水 ( 3 0∶15∶1) ,参比波长 3 2 5nm ,测定波长 2 5 4nm。结果∶两成分分离良好 ,士的宁在 1～ 5 μg、马钱子碱在 0 .5～ 2 .5 μg范围内呈良好的线性关系。平均回收率士的宁为 10 1.0 % ( n =5 ) ,RSD为 3 .2 0 % ,马钱子碱为 10 1.6% (n =5 ) ,RSD为2 .72 %。结论

AIM To develop a method of thin layer chromatographic scanning to determine the content of strychnine and brucine in Maxiecan Capsule.METHODS Silica GF 254 thin layer was used,and the mobile phase consisted of acetone-chloroform-ammonia water(30∶15∶1)with the control wave length of 325nm and detection wave length of 254nm.RESULTS Strychnine and brucine were separated sufficiently and the linear ranges were 1～5μg and 0.5～2.5μg respectively.The mean recovery of strychnine and brucine was 101.0% and 101.6%,with RSD of 3.20% and 2.72% respectively.CONCLUSION This method can be used for the simultaneous determination of strychnine and brucine in Maxiecan capsule.