聚合酶链反应在结核病诊断中的价值

The value of Polymerase chain reachion in diagnosing tuberculosis

目的：为早期诊断结核病寻找一种简便、快速、灵敏的实验室方法。方法：用PCR、结核菌培养、涂片法对临床可疑结核病患者进行检验。结果：在16份痰标本和其它标本中，PCR、结核菌培养、涂片的阳性检出率分别为56．9％、20．7％和7．7％。结论：PCR与其它两种方法比较，差异均非常显著，揭示PCR对结核病的确诊有重要辅助意义。

Objective: To search for a simple and convenient, fast and sensihve laboratory methodon diagnosing tubereulosis earlier. Methods: Test for Mycobacterium tuberculosis by polymerase chainreaction(PCR), culture of bacteria and smear in the dubious tuberculars of clinical diagnosis. Results:detection rates of PCR, culture and smear were 56. 9%, 20. 7 % and 7. 7 % in 16 units of phlegm and theother sampoes. Conclusion: There were all significantly differences in comparing PCR with the othermethods. It indicates that PCR is an important and significative supplementary method to diagnose tu-bereulosis.