人胃癌细胞系和成纤维细胞的 Con A 受体分布的变异

VARIATION IN LOCALIZATION OF CONCANAVALIN A RECEPTORS ON GASTRIC CANCER CELL LINE AND FIBROBLASTS

本文采用 Con A-过氧化物酶光镜和电镜细胞化学方法观察了体外培养的人胃癌细胞(MGC80-3)和成纤维细胞质膜的 Con A 受体分布特点,未经固定的 MGC80-3细胞,Con A 受体复合物呈斑块样不规则分布,而成纤维细胞呈均匀弥散分布。本文还观察了细胞不同的周期时相和生长状态,以及不同的外界因素影响下 Con A 受体复合物的分布变异特点,并对 Con A 受体复合物在胃癌细胞质膜上不规则分布的原因进行了讨论。

The distribution of receptor sites for concanavalin A (Con A) on the cell surface of human gastric cancer cell line (MGC 80-3) and fibroblast cell cultures was studied with electron and light microscope after application of Con A-peroxidase method.The cytochemical reaction of the majority of non-prefixed MGC 80-3 cells shows a striking tendency to be more uneven distribution than on fibroblasts. Clustering,patching and capping of Con A binding sites on the cancer cells and uniform distribution on the fibroblasts were observed.The effects of incubation temperature,prefix treatment,different phase of cell cycles and cell growth condi- tions on the distribution of Con A receptor complexes were observed.The possible reasons for the more irregular distribution of the cytochemical reaction product on the MGC 80-3 cells than the fibroblasts are discussed.