人与几种常用啮齿动物曲细精管周组织超微结构及碱性磷酸酶电镜细胞化学的定位

OBSERVATION OF ULTRASTRUCTURAL AND ALKALINE PHOSPHATASE LOCALIZATION OF THE PERITUBULAR TISSUE OF SEMINIFEROUS TUBULES IN MAN AND SEVERAL RODENTS

本文对人、兔、豚鼠、大鼠及小鼠的曲细精管周组织的超微结构和碱性磷酸酶(AIP)定位进行了观察。人的曲细精管周组织内一般有3层细胞,有细长分支突起,最内层呈肌样细胞特征,向外层的细胞则与成纤维细胞相似。相邻肌样细胞之间多见较宽的细胞间隙。AIP 位于基板、最内侧胶原原纤维区、肌样细胞胞质和向内侧质膜。4种啮齿动物曲细精管周组织微细结构基本相似,但也有某些区别。兔与豚鼠的单层肌样细胞的相邻末端可重叠排列呈2层,而大鼠和小鼠的肌样细胞则呈单层包绕。兔的基板为1～2层,其它3种动物只有1层。4种动物的相邻肌样细胞之间皆有紧密连接与宽度为100～200(?) 的开放间隙,以及更宽的细胞间隙。这4种动物的基板、内非细胞层、肌样细胞胞质及其向内侧质膜和淋巴窦内皮细胞皆有 AIP 分布,除兔以外,豚鼠、大鼠和小鼠的肌样细胞外侧质膜和外非细胞层也有 AIP 分布。本文讨论了曲细精管周组织及 AIP 酶鞘的生理意义。

The ultrastructure and AlP localization of the peritubular tissue of seminiferoustubules in man,rabbits,guinea pigs,rats and mice were observed.Usually,there are three layers of the cell which contains slender and branchcytoplasmic processes in human peritubular tissue.Cells of the inner most layerappear to have the characteristic of myoid cells.The outer layers looked the sameas fibroblasts.The myoid cells are frequently separated by wider intercellular spaces.AlP is localized in the basal lamina,innermost layer of collagenous fibrils zone,cytoplasm and inward plasma membrane of the myoid cells.The ultrastructure of the peritubular tissues of the four rodents are basicallythe same,there are only small differences among them.The myoid cells of rabbit andguinea pig are arranged in one layer and the ends of the adjacent cells may beoverlapped,which have a two-layers appearance.The myoid cells of rat and mouseform just one layer surrounding the tubule.The basal lamina in rabbit appears oneto two layers and in the other three rodents is only one layer.There are tightjunctions,open spaces of 100-200 (?) and wider intercellular spaces between theadjacent myoid cells in these four rodents.The AlP is localized in the basal lamina,inner non-cellular layer,cytoplasm and inward plasma membrane of the myoid cellsas well as endothelial cells of lymphatic sinusoid of all four rodents,but theowtward plasma membrane of myoid cells and outer non-cellular layer have theAlP only in guinea pig,rat,and mouse not in the rabbit.The physiological significance of the peritubular tissue and AlP enzymic sheathwere discussed.