摘要
A new kind of electrophoretic affinity chromatography (EAC) for bioseparation was proposed,Separation by EAC was conducted in a multicompartment electrolyzer in which the affinity gel media were packed in one of the central compartments.The presence of an electric field accelerated the migration of proteins inside the gel matrix during adsorption and descrption processes,This led to the increase of the overall speed of separation,The present study was focused on the effect of the strength of the electric field on adsorption and desorption processes.
A new kind of electrophoretic affinity chromatography (EAC) for bioseparation was proposed. Separation by EAC was conducted in a multicompartment electrolyzer in which the affinity gel media were packed in one of the central compartments. The presence of an electric field accelerated the migration of proteins inside the gel matrix during adsorption and desorption processes. This led to the increase of the overall speed of separation. The present study was focused on the effect of the strength of the electric field on adsorption and desorption processes.
基金
Supported by the State Key Projects(No.96c-03-04-05).
关键词
生物分离
蛋白质
电泳亲合色谱法
EAC
多组分电解剂
吸附
解吸附
affinity chromatography, electrophoresis, electrophoretic affinity chromatography, human serum albumin, Cibacron Blue F3G-A
